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由地中海假单胞菌利用甘油生产可成膜的中链长度聚羟基脂肪酸酯。

Production of filmable medium-chain-length polyhydroxyalkanoates produced from glycerol by Pseudomonas mediterranea.

作者信息

Pappalardo Francesco, Fragalà Manuela, Mineo Placido G, Damigella Arcangelo, Catara Antonino F, Palmeri Rosa, Rescifina Antonio

机构信息

Science and Technology Park of Sicily, Blocco Palma I stradale V. Lancia 57, 95123 Catania, Italy.

Dipartimento di Scienze Chimiche and I.N.S.T.M. UdR of Catania, Università di Catania, Viale A. Doria 6, 95125 Catania, Italy; CNR-IPCF Istituto per i Processi Chimico Fisici, Viale Ferdinando Stagno D'Alcontres 37, 98158 Messina, Italy.

出版信息

Int J Biol Macromol. 2014 Apr;65:89-96. doi: 10.1016/j.ijbiomac.2014.01.014. Epub 2014 Jan 10.

Abstract

Glycerol is an effective carbon source for the production of scl- and mcl-polyhydroxyalkanoates (PHAs) by Pseudomonas spp. P. mediterranea 9.1 (CFBP 5447) synthesizes an amorphous mcl-PHA when grown on crude glycerol, whereas on both reagent grade (RG) and partially refined (PR) glycerol, it produces two very similar distinctive mcl-PHAs with the unusual property of producing, with the appropriate treatment, a transparent film. Mcl-PHAs recovered after biomass extraction have an average molecular weight of approximately 56,000/63,000 Da. The monomer composition and physicochemical properties of such mcl-PHAs suggest their potential application as a softener of biopolymeric blends for food packaging and medical devices.

摘要

甘油是假单胞菌属用于生产短链和中链聚羟基脂肪酸酯(PHA)的有效碳源。地中海假单胞菌9.1(CFBP 5447)在粗甘油上生长时会合成一种无定形中链PHA,而在试剂级(RG)甘油和部分精制(PR)甘油上生长时,它会产生两种非常相似的独特中链PHA,具有经过适当处理可制成透明薄膜的特殊性质。生物质提取后回收的中链PHA平均分子量约为56,000/63,000 Da。此类中链PHA的单体组成和物理化学性质表明它们有潜力作为食品包装和医疗器械生物聚合物共混物的软化剂。

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