嗜麦芽窄食单胞菌TO7利用粗甘油合成中链长度聚羟基脂肪酸酯的特性研究
Characterization of medium-chain-length polyhydroxyalkanoate biosynthesis by Pseudomonas mosselii TO7 using crude glycerol.
作者信息
Liu Ming-Hsu, Chen Yi-Jr, Lee Chia-Yin
机构信息
a Department of Agricultural Chemistry , National Taiwan University , Taipei , Taiwan.
b Department of Nutrition and Health Sciences, Research Center for Industry of Human Ecology and Research Center for Chinese Herbal Medicine, College of Human Ecology , Chang Gung University of Science and Technology , Tao-Yuan , Taiwan.
出版信息
Biosci Biotechnol Biochem. 2018 Mar;82(3):532-539. doi: 10.1080/09168451.2017.1422386. Epub 2018 Jan 17.
Polyhydroxyalkanoates (PHAs) are biopolyesters produced by microorganisms that are environmentally friendly. PHAs can be used to replace traditional plastic to reduce environmental pollution in various fields. PHA production costs are high because PHA must be produced from a carbon substrate. The purpose of this study was to find the strain that can used the BDF by-product as the sole carbon source to produce high amounts of medium-chain-length PHA. Three isolates were evaluated for potential PHA production by using biodiesel-derived crude glycerol as the sole carbon source. Among them, Pseudomonas mosselii TO7 yielded high PHA content. The PHA produced from P. mosselii TO7 were medium-chain-length-PHAs. The PHA content of 48% cell dry weight in 48 h with a maximum PHA productivity of 13.16 mg PHAs L h. The narrow polydispersity index value of 1.3 reflected the homogeneity of the polymer chain, which was conducive to industrial applications.
聚羟基脂肪酸酯（PHA）是由微生物产生的对环境友好的生物聚酯。PHA可用于替代传统塑料，以减少各个领域的环境污染。PHA的生产成本很高，因为PHA必须由碳底物生产。本研究的目的是找到能够将生物柴油副产物作为唯一碳源来大量生产中链长度PHA的菌株。通过使用生物柴油衍生的粗甘油作为唯一碳源，对三株分离菌进行了PHA生产潜力评估。其中，莫氏假单胞菌TO7产生的PHA含量很高。从莫氏假单胞菌TO7产生的PHA是中链长度的PHA。在48小时内PHA含量为细胞干重的48%，最大PHA生产率为13.16毫克PHA/升·小时。1.3的窄多分散指数值反映了聚合物链的均匀性，这有利于工业应用。
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