Sinha A A, Wilson M J, Gleason D F
Cancer. 1987 Sep 15;60(6):1288-93. doi: 10.1002/1097-0142(19870915)60:6<1288::aid-cncr2820600621>3.0.co;2-v.
Human prostate specimens from 25 patients provided 15 normal, four benign prostatic hypertrophy (BPH), and seven adenocarcinoma samples which were studied using a polyclonal antibody against prostatic specific antigen (PSA) and protein A-gold complex. Our study showed the presence of gold particles in cytoplasmic vesicles and granules, rough endoplasmic reticulum, and occasional lysosomal dense bodies in columnar or cuboidal cells of acini from normal, BPH and well-differentiated cancerous specimens, but not in the acinar basal cells. Some moderately differentiated and most poorly differentiated tumors contained undifferentiated neoplastic cells in which PSA localizations often were associated with membranous structures since specific cytoplasmic organelles were not differentiated. In prostatic stroma, invasive cells which were of differentiated type localized gold particles. In addition, some neutrophils and macrophages also localized PSA suggesting their role in phagocytosis of extracellularly released PSA in the stroma of BPH and cancerous samples. Thus far, the authors have not observed neutrophils and macrophages with gold particles in normal prostate. We suggest that neutrophils and macrophages were involved in transport of some extracellularly released PSA from the prostatic stroma or other metastatic sites to sera in BPH and cancer patients.
从25名患者的前列腺标本中获取了15份正常样本、4份良性前列腺增生（BPH）样本和7份腺癌样本，使用抗前列腺特异性抗原（PSA）的多克隆抗体和蛋白A-金复合物对这些样本进行了研究。我们的研究表明，在正常、BPH和高分化癌标本的腺泡柱状或立方体细胞的胞质囊泡、颗粒、粗面内质网以及偶尔的溶酶体致密小体中存在金颗粒，但在腺泡基底细胞中未发现。一些中分化和大多数低分化肿瘤含有未分化的肿瘤细胞，由于特定的细胞质细胞器未分化，PSA定位通常与膜结构相关。在前列腺基质中，分化型的浸润细胞定位有金颗粒。此外，一些中性粒细胞和巨噬细胞也定位有PSA，表明它们在BPH和癌样本基质中吞噬细胞外释放的PSA中发挥作用。到目前为止，作者在正常前列腺中未观察到带有金颗粒的中性粒细胞和巨噬细胞。我们认为，中性粒细胞和巨噬细胞参与了将BPH和癌症患者前列腺基质或其他转移部位细胞外释放的一些PSA转运至血清的过程。
