Gallee M P, Visser-de Jong E, van der Korput J A, van der Kwast T H, ten Kate F J, Schroeder F H, Trapman J
Department of Pathology, Erasmus University, Rotterdam, The Netherlands.
Urol Res. 1990;18(3):181-7. doi: 10.1007/BF00295844.
A series of 55 randomly chosen radical prostatectomy specimens was analyzed for expression of prostate-specific antigen (PSA) by immunohistochemical techniques. Tissue sections were selected in such a manner that in addition to glandular benign prostatic hyperplasia (BPH), one or more different prostatic tumour growth patterns were present. Four monoclonal antibodies, directed against three different PSA epitopes, and one polyclonal anti-PSA antiserum were used. Expression of PSA was compared with that of prostate-specific acid phosphatase (PAP), recognized by two different polyclonal antisera. A critical dilution aimed at a maximum of staining intensity on BPH tissue sections was chosen for all antibodies. Anti-PSA and anti-PAP antisera stained essentially all BPH samples (over 90%). Irrespective of the nature of the antibodies used, PSA expression was found to be decreased in prostatic carcinoma. A clear cut relationship was found between immunoreactivity for PSA and the degree of differentiation of the tumour area. Under the experimental conditions used the PSA monoclonal antibodies stained only 1 out of 10 undifferentiated carcinomas, whereas 50% to 70% of the well- and moderately-differentiated carcinomas showed immunoreactivity. This correlation was less pronounced with the PAP staining pattern. If the PSA antibody titer was raised the percentage of clearly staining undifferentiated carcinomas could be considerably increased (up to 60%-100%), indicating that PSA expression is not absent, but lowered in most (if not all) undifferentiated carcinomas.
通过免疫组织化学技术对55例随机选取的根治性前列腺切除术标本进行分析，以检测前列腺特异性抗原（PSA）的表达。组织切片的选取方式使得除了腺性良性前列腺增生（BPH）外，还存在一种或多种不同的前列腺肿瘤生长模式。使用了针对三种不同PSA表位的四种单克隆抗体以及一种多克隆抗PSA抗血清。将PSA的表达与两种不同多克隆抗血清识别的前列腺特异性酸性磷酸酶（PAP）的表达进行比较。为所有抗体选择了一个临界稀释度，旨在使BPH组织切片上的染色强度达到最大。抗PSA和抗PAP抗血清对基本上所有BPH样本（超过90%）都有染色。无论所用抗体的性质如何，在前列腺癌中都发现PSA表达降低。在PSA的免疫反应性与肿瘤区域的分化程度之间发现了明确的关系。在所使用的实验条件下，PSA单克隆抗体在10例未分化癌中仅对1例有染色，而50%至70%的高分化和中分化癌显示有免疫反应性。这种相关性在PAP染色模式中不太明显。如果提高PSA抗体滴度，明显染色的未分化癌的百分比会大幅增加（高达60%-100%），这表明在大多数（如果不是全部）未分化癌中PSA表达并非不存在，而是降低了。
