Hansen J E, Iversen J, Lihme A, Bøg-Hansen T C
Cancer. 1987 Oct 1;60(7):1630-5. doi: 10.1002/1097-0142(19871001)60:7<1630::aid-cncr2820600735>3.0.co;2-x.
The acute phase proteins, orosomucoid, ceruloplasmin, antitrypsin, and haptoglobin were measured in serum from 54 patients with lung cancer, 16 patients with benign lung inflammation, and 30 healthy individuals. A statistical correlation was found between tumor size and acute phase protein level, which, however, was ascribed to nonspecific inflammation in the tissues surrounding the tumor. The patients who subsequently could not be radically treated by surgery had higher concentrations of orosomucoid and ceruloplasmin than the radically treated patients. No difference in acute phase protein concentration was found between benign and malignant disease. The glycan-dependent microheterogeneity of orosomucoid and ceruloplasmin was analyzed by crossed affinoimmunoelectrophoresis with lectins, and the patterns of the patients with benign inflammation and malignant disease were different. The heterogeneity of ceruloplasmin was also analyzed by crossed immunoelectrophoresis without lectin. This analysis, combined with the total serum concentration of ceruloplasmin, made it possible to discriminate the 54 cases of malignancy from the 46 cases of nonmalignancy with a sensitivity of 78% and a specificity of 93%. It is suggested that the simple electrophoretic analyses of (micro-)heterogeneity is a valuable supplement to the acute phase profile in isolating high-risk patients and in monitoring radically treated cancer patients for relapse.
对54例肺癌患者、16例良性肺部炎症患者和30名健康个体的血清进行了急性期蛋白、血清类粘蛋白、铜蓝蛋白、抗胰蛋白酶和触珠蛋白的检测。发现肿瘤大小与急性期蛋白水平之间存在统计学相关性，但这归因于肿瘤周围组织的非特异性炎症。随后无法通过手术进行根治性治疗的患者血清类粘蛋白和铜蓝蛋白浓度高于接受根治性治疗的患者。良性和恶性疾病之间急性期蛋白浓度没有差异。通过与凝集素的交叉亲和免疫电泳分析了血清类粘蛋白和铜蓝蛋白的聚糖依赖性微异质性，良性炎症患者和恶性疾病患者的模式不同。还通过无凝集素的交叉免疫电泳分析了铜蓝蛋白的异质性。该分析结合铜蓝蛋白的血清总浓度，能够以78%的敏感性和93%的特异性区分54例恶性肿瘤病例和46例非恶性肿瘤病例。有人认为，（微）异质性的简单电泳分析是急性期指标的有价值补充，可用于识别高危患者以及监测接受根治性治疗的癌症患者是否复发。
