来自C型肉毒杆菌祖毒素的HA17-HA70（HA2-HA3）复合物的纯化、结晶及初步X射线分析
Purification, crystallization and preliminary X-ray analysis of an HA17-HA70 (HA2-HA3) complex from Clostridium botulinum type C progenitor toxin.
作者信息
Iwasa Chikako, Tonozuka Takashi, Shinoda Masaya, Sagane Yoshimasa, Niwa Koichi, Watanabe Toshihiro, Yoshida Hiromi, Kamitori Shigehiro, Takao Toshifumi, Oguma Keiji, Nishikawa Atsushi
机构信息
Department of Applied Biological Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan.
Department of Food and Cosmetic Science, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri 099-2493, Japan.
出版信息
Acta Crystallogr F Struct Biol Commun. 2014 Jan;70(Pt 1):64-7. doi: 10.1107/S2053230X13032378. Epub 2013 Dec 24.
The haemagglutinin (HA) complex of Clostridium botulinum type C toxin is composed of three types of subcomponents: HA33, HA17 and HA70 (also known as HA1, HA2 and HA3, respectively). Here, a 260 kDa HA17-HA70 complex was crystallized. His-tagged HA17 and maltose-binding-protein-tagged HA70 were expressed in Escherichia coli and their complex was affinity-purified using a combination of amylose resin chromatography and nickel-nitrilotriacetic acid agarose chromatography. Diffraction data were collected to 8.0 Å resolution and the crystal belonged to the tetragonal space group P4(1)2(1)2. The molecular-replacement solution indicated that one molecule of HA17 was bound to each HA70 monomer.
C型肉毒杆菌毒素的血凝素（HA）复合物由三种亚组分组成：HA33、HA17和HA70（也分别称为HA1、HA2和HA3）。在此，一个260 kDa的HA17-HA70复合物被结晶。带有His标签的HA17和带有麦芽糖结合蛋白标签的HA70在大肠杆菌中表达，它们的复合物通过直链淀粉树脂色谱和镍-次氮基三乙酸琼脂糖色谱联用进行亲和纯化。收集到了分辨率为8.0 Å的衍射数据，晶体属于四方晶系空间群P4(1)2(1)2。分子置换结果表明，一个HA17分子与每个HA70单体结合。
Zotero 插件