Nakamura Toshio, Tonozuka Takashi, Kotani Mao, Obata Kanae, Oguma Keiji, Nishikawa Atsushi
Department of Applied Biological Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Dec 1;63(Pt 12):1038-40. doi: 10.1107/S1744309107053973. Epub 2007 Nov 21.
HA3, a 70 kDa haemagglutinating protein, is a precursor form of HA3a and HA3b, the subcomponents of Clostridium botulinum type C 16S progenitor toxin. In this report, recombinant HA3 protein was overexpressed in Escherichia coli, purified and crystallized. Diffraction data were collected to 2.6 A resolution and the crystal belonged to the hexagonal space group P6(3). Matthews coefficient and self-rotation function calculations indicate that there is probably one molecule of HA3 in the asymmetric unit. A search for heavy-atom derivatives has been undertaken.
HA3是一种70 kDa的血凝蛋白,是肉毒杆菌C型16S祖毒素亚组分HA3a和HA3b的前体形式。在本报告中,重组HA3蛋白在大肠杆菌中过量表达、纯化并结晶。收集了分辨率为2.6 Å的衍射数据,晶体属于六方空间群P6(3)。马修斯系数和自旋转函数计算表明,不对称单元中可能有一个HA3分子。已对重原子衍生物进行了搜索。