一种简单的方法可用于鉴定肉毒梭菌菌落。

A simple procedure for identification ofClostridium botulinum colonies.

机构信息

Department of Medical Laboratory Sciences, College of Health Florida International University, 33199, Miami, FL, USA.

出版信息

World J Microbiol Biotechnol. 1993 Jan;9(1):125-7. doi: 10.1007/BF00656534.

PMID:24419857

Abstract

For direct identification of toxigenic colonies ofClostridium botulinum type E, suspected colonies are uniformly suspended in a phosphate buffer containing 0.5% (w/v) gelatin and 0.05% (w/v) Tween 20. After centrifuging, the supernatant is tested for botulinal toxin by an enzyme-linked immunosorbent assay (ELISA). The assay is specific for this type as it did not react with culture filtrates of otherClostridium species, including non-toxigenic 'E-like' organisms.

摘要

为了直接鉴定 E 型肉毒梭菌的产毒菌落，将可疑菌落均匀悬浮在含有 0.5%（w/v）明胶和 0.05%（w/v）吐温 20 的磷酸盐缓冲液中。离心后，通过酶联免疫吸附测定（ELISA）检测上清液中的肉毒毒素。该检测方法具有特异性，因为它不会与其他梭菌属的培养滤液发生反应，包括非产毒的“E 样”生物体。