Dezfulian M, Bartlett J G
Department of Medical Laboratory Sciences, Florida International University, Miami 33199.
Diagn Microbiol Infect Dis. 1991 Jan-Feb;14(1):11-5. doi: 10.1016/0732-8893(91)90080-y.
A sensitive double-sandwich enzyme immunoassay for identification of toxigenic Clostridium botulinum type E was developed with a type-E-specific antitoxin. The antitoxin was produced in a rabbit, following inoculation of minute quantities of type-E toxoid. The toxoid was prepared from a commercially available toxin by using a detoxification method. Cross-reactivity of the antitoxin with C. botulinum types A and B was avoided by absorption of the serum with alum-precipitated toxoids A and B. The immunoassay proved to be specific for C. botulinum type E because it did not react with culture filtrates of various Clostridium species, including nontoxigenenic "E-like" organisms and Clostridium sporogenes.
利用E型特异性抗毒素开发了一种用于鉴定产毒E型肉毒梭菌的灵敏双夹心酶免疫测定法。通过给兔子接种微量E型类毒素来制备抗毒素。类毒素是采用解毒方法从市售毒素制备而来。通过用明矾沉淀的A和B型类毒素吸收血清，避免了抗毒素与A型和B型肉毒梭菌的交叉反应。该免疫测定法被证明对E型肉毒梭菌具有特异性，因为它不与各种梭菌属的培养滤液发生反应，包括不产毒的“E样”菌和生孢梭菌。
