Hayes S
Infect Immun. 1979 Oct;26(1):150-6. doi: 10.1128/iai.26.1.150-156.1979.
Clostridium botulinum neurotoxin is synthesized by toxic clones grown anaerobically on ganglioside affinity filters. The toxin binds to the filters and is detected by reaction with 125I-immunoglobulin G from type-specific antitoxin. Toxin spots from culture filtrates were similarly identified. The C. botulinum type C and D strains were selected for developing this affinity filter assay because synthesis of the C1 and D toxins is bacteriophage dependent. Toxigenic clones were distinguished from prophage-cured atoxigenic derivatives. These studies represent a first step toward the development of a general nonbiological screening procedure for identifying botulinal toxin and toxigenic cells. The affinity filter methodology should facilitate genetic analysis of the basis of C. botulinum toxicity.
肉毒梭菌神经毒素由在神经节苷脂亲和滤膜上厌氧培养的产毒克隆合成。毒素与滤膜结合，并通过与来自型特异性抗毒素的125I-免疫球蛋白G反应进行检测。培养滤液中的毒素斑点也以类似方式鉴定。选择肉毒梭菌C型和D型菌株来开发这种亲和滤膜检测方法，因为C1和D毒素的合成依赖于噬菌体。产毒克隆与经噬菌体治愈的无毒衍生物得以区分。这些研究是朝着开发一种用于鉴定肉毒毒素和产毒细胞的通用非生物学筛选程序迈出的第一步。亲和滤膜方法应有助于对肉毒梭菌毒性基础进行遗传分析。
