测定丙酮酸、磷酸烯醇式丙酮酸二激酶活性：使用磷酸烯醇丙酮酸羧激酶作为偶联酶时的必要注意事项。
Assaying for pyruvate, orthophosphate dikinase activity: Necessary precautions with phosphoenolpyruvate carboxylase as coupling enzyme.
机构信息
Laboratory of Plant Physiology, Department of Biology, University of Patras, Patras, Greece.
出版信息
Photosynth Res. 1990 May;24(2):183-8. doi: 10.1007/BF00032598.
Phosphoenolpyruvate carboxylase (EC 4.1.1.31), used as a coupling enzyme in the assay of the pyruvate, orthophosphate dikinase (EC 2.7.9.1) forward reaction, is a serious limiting factor for the overall rate when added at a level of 0.2-0.3 unit/ml of assay medium. Nonlimiting assay conditions are obtained by either increasing the level of the coupling enzyme to 3 units/ml or adding 6mM glucose-6-phosphate as an activator/stabilizer of phosphoenolpyruvate carboxylase.
磷酸烯醇式丙酮酸羧化酶（EC 4.1.1.31），用作测定丙酮酸-正磷酸二激酶（EC 2.7.9.1）正向反应的偶联酶，当添加到测定介质的 0.2-0.3 单位/ml 水平时，是整体速率的严重限制因素。通过将偶联酶的水平提高到 3 单位/ml 或添加 6mM 葡萄糖-6-磷酸作为磷酸烯醇式丙酮酸羧化酶的激活剂/稳定剂，可以获得非限制测定条件。
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