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Biochem J. 1969 Aug;114(1):117-25. doi: 10.1042/bj1140117.
2
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Characterization of the covalent enzyme intermediates formed during pyruvate phosphate dikinase catalysis.丙酮酸磷酸二激酶催化过程中形成的共价酶中间体的表征。
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7
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8
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9
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Sci Rep. 2017 Aug 14;7(1):8020. doi: 10.1038/s41598-017-08521-w.
2
Effects of glycerol on the in vitro stability and regulatory activation/inactivation of pyruvate, orthophosphate dikinase of Zea mays L.甘油对玉米丙酮酸，磷酸二激酶体外稳定性和调节激活/失活的影响。
Photosynth Res. 1990 Oct;26(1):9-17. doi: 10.1007/BF00048972.
3
Assaying for pyruvate, orthophosphate dikinase activity: Necessary precautions with phosphoenolpyruvate carboxylase as coupling enzyme.测定丙酮酸、磷酸烯醇式丙酮酸二激酶活性：使用磷酸烯醇丙酮酸羧激酶作为偶联酶时的必要注意事项。
Photosynth Res. 1990 May;24(2):183-8. doi: 10.1007/BF00032598.
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Planta. 1980 Feb;147(5):477-84. doi: 10.1007/BF00380191.
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Pyrophosphate-dependent fructose-6-phosphate 1-phosphotransferase induction and attenuation of Hsp gene expression during endosperm modification in quality protein maize.在优质蛋白玉米胚乳修饰过程中，焦磷酸依赖性果糖-6-磷酸 1-磷酸转移酶的诱导和热休克蛋白基因表达的衰减。
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A Kinetic Characterization of Slow Inactivation of Ribulosebisphosphate Carboxylase during Catalysis.在催化过程中测定核酮糖二磷酸羧化酶的缓慢失活的动力学特征。
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Plant Physiol. 1982 Feb;69(2):371-4. doi: 10.1104/pp.69.2.371.
8
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Plant Physiol. 1982 Jan;69(1):7-10. doi: 10.1104/pp.69.1.7.
9
Isolation of Intact and Functional Chloroplasts from Mesophyll and Bundle Sheath Protoplasts of the C(4) Plant Panicum miliaceum.从C4植物黍的叶肉和维管束鞘原生质体中分离完整且有功能的叶绿体
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10
Plants under Climatic Stress: VI. Chilling and Light Effects on Photosynthetic Enzymes of Sorghum and Maize.遭受气候胁迫的植物：VI. 低温和光照对高粱和玉米光合酶的影响
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本文引用的文献

1
The chromatographic identification of some biologically important phosphate esters.某些具有重要生物学意义的磷酸酯的色谱鉴定
J Biol Chem. 1951 Nov;193(1):405-10.
2
The participation of phosphoenolpyruvate synthetase in photosynthetic CO2 fixation of tropical grasses.磷酸烯醇丙酮酸合成酶在热带禾本科植物光合二氧化碳固定中的作用。
Arch Biochem Biophys. 1967 Apr;120(1):224-5. doi: 10.1016/0003-9861(67)90618-2.
3
Photosynthesis by sugar-cane leaves. A new carboxylation reaction and the pathway of sugar formation.甘蔗叶片的光合作用。一种新的羧化反应和糖分形成途径。
Biochem J. 1966 Oct;101(1):103-11. doi: 10.1042/bj1010103.
4
Isotope exchange studies of the mechanism of the reaction catalyzed by adenosine triphosphate: creatine phosphotransferase.三磷酸腺苷：肌酸磷酸转移酶催化反应机制的同位素交换研究。
J Biol Chem. 1966 Feb 10;241(3):673-83.
5
Studies on the mechanism of activation and inactivation of pyruvate, phosphate dikinase. A possible regulatory role for the enzyme in the C4 dicarboxylic acid pathway of photosynthesis.丙酮酸、磷酸二激酶的激活与失活机制研究。该酶在光合作用的C4二羧酸途径中可能的调节作用。
Biochem J. 1969 May;112(5):549-58. doi: 10.1042/bj1120549.
6
A new enzyme for the interconversion of pyruvate and phosphopyruvate and its role in the C4 dicarboxylic acid pathway of photosynthesis.一种用于丙酮酸和磷酸丙酮酸相互转化的新酶及其在光合作用C4二羧酸途径中的作用。
Biochem J. 1968 Jan;106(1):141-6. doi: 10.1042/bj1060141.
7
The pyruvate-phosphate dikinase reaction. The fate of phosphate and the equilibrium.丙酮酸磷酸双激酶反应。磷酸的去向与平衡
J Biol Chem. 1968 Oct 25;243(20):5486-91.
8
A new enzyme with the glycolytic function of pyruvate kinase.一种具有丙酮酸激酶糖酵解功能的新酶。
J Biol Chem. 1968 Jun 10;243(11):3202-4.
9
Comparative studies on the activity of carboxylases and other enzymes in relation to the new pathway of photosynthetic carbon dioxide fixation in tropical grasses.热带禾本科牧草中羧化酶及其他酶的活性与光合二氧化碳固定新途径的比较研究
Biochem J. 1967 Jun;103(3):660-5. doi: 10.1042/bj1030660.
10
An improved method for the determination of orthophosphate suitable for assay of adenosine triphosphatase activity.一种改进的用于测定正磷酸盐的方法，适用于腺苷三磷酸酶活性的测定。
Anal Chem. 1966 Aug;38(9):1182-7. doi: 10.1021/ac60241a015.

来自叶片的丙酮酸磷酸双激酶的性质及作用机制

Properties and mechanism of action of pyruvate, phosphate dikinase from leaves.

作者信息

Andrews T J, Hatch M D

出版信息

Biochem J. 1969 Aug;114(1):117-25. doi: 10.1042/bj1140117.

DOI:10.1042/bj1140117
PMID:5810041
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1184803/
Abstract
  1. Sugar-cane leaf pyruvate,P(i) dikinase was prepared free of enzymes that would interfere with studies on the stoicheiometry and mechanism of the reaction it catalyses. The reaction was unequivocally shown to involve the conversion of equimolar amounts of pyruvate, ATP and P(i) into phosphoenolpyruvate, AMP and PP(i). 2. The purified enzyme was stable at pH8.3 only if stored at about 20 degrees in the presence of Mg(2+) and a thiol-reducing reagent, care being taken to prevent the oxidation of the thiol. 3. The apparent Michaelis constants for phosphoenolpyruvate and PP(i) were 0.11mm and 0.04mm respectively and that for AMP was less than 4mum. 4. At pH8.3 the initial velocity of the reaction was about 6 times as fast in the direction towards phosphoenolpyruvate synthesis as in the reverse direction. 5. With the exception of ATP, all the products of the reaction in both directions were inhibitory. 6. The phosphate groups of PP(i) were derived from P(i) and from the terminal phosphate of ATP. 7. Isotope-exchange studies indicated that the reaction proceeds in the following steps:Enzyme+ATP+P(i) right harpoon over left harpoon Enzyme-P+AMP+PP(i)Enzyme-P+pyruvate right harpoon over left harpoon Enzyme+phosphoenolpyruvate
摘要

  1. 制备了甘蔗叶丙酮酸、磷酸（Pi）二激酶，使其不含会干扰对其催化反应的化学计量和机制研究的酶。明确表明该反应涉及等摩尔量的丙酮酸、ATP和磷酸（Pi）转化为磷酸烯醇丙酮酸、AMP和焦磷酸（PPi）。

  2. 纯化后的酶仅在pH8.3时稳定，前提是在镁离子（Mg2+）和一种硫醇还原试剂存在的情况下于约20摄氏度储存，要注意防止硫醇氧化。

  3. 磷酸烯醇丙酮酸和焦磷酸（PPi）的表观米氏常数分别为0.11毫摩尔和0.04毫摩尔，而AMP的表观米氏常数小于4微摩尔。

  4. 在pH8.3时，反应朝着磷酸烯醇丙酮酸合成方向的初始速度约为反向的6倍。

  5. 除了ATP外，反应在两个方向上的所有产物均具有抑制作用。

  6. 焦磷酸（PPi）的磷酸基团来自磷酸（Pi）和ATP的末端磷酸基团。

  7. 同位素交换研究表明反应按以下步骤进行：酶+ATP+磷酸（Pi）⇌酶-P+AMP+焦磷酸（PPi）；酶-P+丙酮酸⇌酶+磷酸烯醇丙酮酸