Department of Orthopaedics, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, 510010, People's Republic of China,
J Mater Sci Mater Med. 2014 Mar;25(3):801-12. doi: 10.1007/s10856-013-5115-y. Epub 2014 Jan 14.
In this study, the scaffolds based on mineralized silver-loaded coral hydroxyapatites (SLCHAs) were developed for bone regeneration in the radius of rabbit with a 15-mm infective segmental defect model for the first time. The SLCHAs were achieved by surface adsorption and ion-exchange reaction between Ca(2+) of coral hydroxyapatite (CHA) and Ag(+) of silver nitrate with different concentration at room temperature. Release experiment in vitro, X-ray diffraction and scanning electron microscopy equipped with energy-dispersive X-ray spectrometer were applied to exhibit that the scaffold showed some features of natural bone both in main component and hierarchical microstructure. The three-dimensional porous scaffold materials imitate the microstructure of cancellous bone. Mouse embryonic pre-osteoblast cells (MC3T3-E1) were used to investigate the cytocompatibility of SLCHAs, CHA and pure coral. Cell activity were studied with alkaline phosphataseenzyme assay after 2, 4, 6 days of incubation. It was no statistically significant differences in cell activity on the scaffolds of Ag(+)(13.6 μg/mL)/CHA, Ag(+)(1.7 μg/mL)/CHA, CHA and pure coral. The results indicated that the lower silver concentration has little effect on cell activity. In the implantation test, the infective segmental defect repaired with SLCHAs was healed up after 10 weeks after surgery, and the implanted composites were almost substituted by new bone tissue, which were very comparable with the scaffold based on mineralized CHA. It could be concluded that the SLCHAs contained with appropriate silver ionic content could act as biocidal agents and maintain the advantages of mineralized CHA or coral, while avoiding potential bacteria-dangers and toxical heavy-metal reaction. All the above results showed that the SLCHAs with anti-infective would be as a promising scaffold material, which whould be widely applied into the clinical for bone regeneration.
在这项研究中，首次基于载银矿化珊瑚羟基磷灰石（SLCHA）的支架被开发用于兔桡骨 15mm 感染性节段性缺损模型的骨再生。通过室温下珊瑚羟基磷灰石（CHA）中的 Ca（2+）与硝酸银中的 Ag（+）之间的表面吸附和离子交换反应，实现了 SLCHA。体外释放实验、X 射线衍射和扫描电子显微镜（配备能量色散 X 射线光谱仪）表明，支架在主要成分和分级微观结构上均具有天然骨的一些特征。三维多孔支架材料模仿松质骨的微观结构。使用鼠胚胎前成骨细胞（MC3T3-E1）来研究 SLCHA、CHA 和纯珊瑚的细胞相容性。用碱性磷酸酶酶法测定细胞活力，在孵育 2、4、6 天后进行研究。Ag（+）（13.6μg/mL）/CHA、Ag（+）（1.7μg/mL）/CHA、CHA 和纯珊瑚支架上的细胞活性没有统计学上的显著差异。结果表明，较低的银浓度对细胞活性影响不大。在植入试验中，植入 SLCHA 的感染性节段性缺损在手术后 10 周后愈合，植入的复合材料几乎被新骨组织替代，与矿化 CHA 为基础的支架非常相似。可以得出结论，含有适当银离子含量的 SLCHA 可以作为杀菌剂，并保持矿化 CHA 或珊瑚的优势，同时避免潜在的细菌危险和有毒重金属反应。所有上述结果表明，具有抗感染作用的 SLCHA 将成为一种有前途的支架材料，将广泛应用于骨再生的临床。
