对热释光、延迟发光和光合材料产氧的研究：紫外光效应。

Studies on thermoluminescence, delayed light emission and oxygen evolution from photosynthetic materials: UV effects.

机构信息

Molecular Biology and Agriculture Division, Bhabha Atomic Research Centre, Trombay, 400 085, Bombay, India.

出版信息

Photosynth Res. 1990 Jul;25(1):17-24. doi: 10.1007/BF00051732.

PMID:24420167

Abstract

The effect of ultraviolet light on thermoluminescence, oxygen evolution and the slow component of delayed light has been investigated in chloroplasts and Pothos leaves. All peaks including peak V (48°C) were inhibited by UV. However, the peak at 48°C which was induced by DCMU was enhanced following UV irradiation of chloroplasts at ambient temperature (23°C) whereas peak II (-12°C) and peak III (10°C) which were also induced by DCMU were inhibited. Chloroplasts treated with DCMU and dark incubated for several minutes at ambient temperature prior to recording of glow curves have also shown enhancement of peak at 48°C. A slow component of delayed light and photosystem II activity of chloroplasts were inhibited by UV whereas photosystem I activity was marginally affected. These results corroborate involvement of photosystem II in generating thermoluminescence and slow components of delayed light in photosynthetic materials.

摘要

研究了紫外线对叶绿体和绿萝叶片中的热释光、氧释放和延迟光慢组分的影响。所有峰，包括峰 V（48°C），均被 UV 抑制。然而，在室温（23°C）下用 UV 辐照叶绿体时，DCMU 诱导的 48°C 峰增强，而 DCMU 也诱导的峰 II（-12°C）和峰 III（10°C）被抑制。用 DCMU 处理叶绿体并在室温下暗孵育几分钟，然后记录发光曲线，也显示出 48°C 峰的增强。紫外线抑制了叶绿体的延迟光慢组分和光系统 II 的活性，而光系统 I 的活性则略有影响。这些结果证实了光系统 II 参与了光合材料中热释光和延迟光慢组分的产生。