Ichikawa T, Inoue Y, Shibata K
Biochim Biophys Acta. 1975 Dec 11;408(3):228-39. doi: 10.1016/0005-2728(75)90126-7.
Plant materials (intact leaves, chloroplasts or subchloroplast particles) pre-illuminated at a low temperature (e.g. -60 degrees C) were rapidly cooled to -196 degrees C and then the luminescence emitted from the sample on raising the temperature was measured as a function of temperature, by means of a sensitive photo-electron counting technique. Mature spinach leaves showed five luminescence bands at different temperatures which were denoted as ZV, A, B1, B2 and C bands. The A, B1, B2 and C bands appeared at constant temperatures, -10, +25, +40 and +55 degrees C, respectively, being independent of the illumination temperature, but the ZV band appeared at a variable temperature slightly higher than the illumination temperature. The B1 and B2 bands were absent in the thermoluminescence profiles of samples devoid of the oxygen-evolving activity, such as heat-treated spinach leaves, wheat leaves greened under intermittent illumination and photosystem-II particles prepared with Triton X-100. It was deduced that these luminescence bands arise from the energy stored by the electron flow in photosystem II to evolve oxygen, and other bands were ascribed to charge-separation in some other sites not related to the oxygen evolving system.
在低温（如-60℃）下预光照的植物材料（完整叶片、叶绿体或亚叶绿体颗粒）迅速冷却至-196℃，然后通过灵敏的光电子计数技术，测量样品升温时发出的发光强度随温度的变化。成熟菠菜叶在不同温度下呈现出五条发光带，分别标记为ZV、A、B1、B2和C带。A、B1、B2和C带分别出现在恒定温度-10℃、+25℃、+40℃和+55℃，与光照温度无关，但ZV带出现在略高于光照温度的可变温度下。在缺乏放氧活性的样品的热发光图谱中，如热处理的菠菜叶、间歇光照下变绿的小麦叶以及用Triton X-100制备的光系统II颗粒，不存在B1和B2带。据推断，这些发光带源于光系统II中电子流为放氧而储存的能量，其他带则归因于与放氧系统无关的其他位点的电荷分离。
