Pohl C, Baroudy B M, Bergmann K F, Cote P J, Purcell R H, Hoofnagle J, Gerin J L
J Infect Dis. 1987 Oct;156(4):622-9. doi: 10.1093/infdis/156.4.622.
Peripheral blood lymphocytes from a patient chronically infected with hepatitis D virus (HDV) were immortalized by Epstein-Barr virus transformation. Two stable monoclonal cell lines, derived from the same parent culture, were established and produced antibodies of the IgG isotype that were specific for the hepatitis delta antigen (HDAg). Both monoclonal antibodies (MAbs) recognized the major HDAg polypeptides of 24 kilodaltons and 27 kilodaltons that were previously detected by polyclonal antibodies to HDAg in both liver and serum from HDV-infected humans, chimpanzees, and woodchucks. This result indicates that the major polypeptides of HDAg share common epitopes. The MAbs also reacted with minor polypeptides of lower molecular weight, which were present in infected liver. In vitro translation products of HDV-specific RNA from infected liver were also detected by the MAbs; these polypeptides were 24 kilodaltons and 27 kilodaltons, respectively, and comigrated with liver- or serum-derived HDAg. In contrast, HDV RNA isolated from virions in serum was not translated into HDAg polypeptides in the in vitro system.
通过爱泼斯坦 - 巴尔病毒转化使一名慢性感染丁型肝炎病毒（HDV）患者的外周血淋巴细胞永生化。从同一亲本培养物中获得了两个稳定的单克隆细胞系，它们产生了IgG同种型抗体，这些抗体对丁型肝炎抗原（HDAg）具有特异性。两种单克隆抗体（MAb）都识别24千道尔顿和27千道尔顿的主要HDAg多肽，这些多肽先前已在HDV感染的人类、黑猩猩和土拨鼠的肝脏和血清中被抗HDAg多克隆抗体检测到。这一结果表明HDAg的主要多肽具有共同的表位。这些MAb还与感染肝脏中存在的较低分子量的次要多肽发生反应。MAb也检测到了来自感染肝脏的HDV特异性RNA的体外翻译产物；这些多肽分别为24千道尔顿和27千道尔顿，并且与肝脏或血清来源的HDAg迁移率相同。相比之下，从血清中的病毒粒子分离出的HDV RNA在体外系统中未被翻译成HDAg多肽。
