Doyle G, Everhart D, Mallett C, Ayakawa G, Bleiweis A S
J Gen Microbiol. 1986 Oct;132(10):2885-92. doi: 10.1099/00221287-132-10-2885.
Monoclonal antibodies (MAb) raised to intact Streptococcus mutans P-4 cells (serotype e) were used to demonstrate the presence of shared antigenic determinant(s) between S. mutans BHT (serotype b) cell membranes and human heart tissue. MAb binding to both BHT membrane and human heart tissue was demonstrated by ELISA. Common antigens were identified by immunoblot analysis following separation of BHT membrane components and human heart antigens by SDS-PAGE. MAb 22C4 recognized three polypeptides from the BHT membrane preparation, having molecular masses of 42, 56 and 85 kDa. MAb 22C4 also recognized an 85 kDa component and a 200 kDa component from human heart tissue. MAb D159 was specific for a single 82 kDa polypeptide in BHT membrane, and also bound to two high molecular mass components in human heart (165 and 200 kDa). When both MAb D159 and 22C4 were first absorbed with S. mutans P-4 cells, subsequent reactivity to the aforementioned BHT membrane components was inhibited, indicating that these cross-reactive components are found in S. mutans P-4 as well as in S. mutans BHT micro-organisms. Competitive binding analysis showed that both MAb D159 and MAb 22C4 bound to myosin, indicating that S. mutans BHT membrane, human heart tissue and myosin share at least one immunodeterminant. This indicates that myosin could be the cross-reactive tissue component in human heart.
用针对完整变形链球菌P-4细胞（血清型e）产生的单克隆抗体（MAb）来证明变形链球菌BHT（血清型b）细胞膜与人心脏组织之间存在共同抗原决定簇。通过酶联免疫吸附测定（ELISA）证明了MAb与BHT膜和人心脏组织的结合。在通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分离BHT膜成分和人心脏抗原后，通过免疫印迹分析鉴定了共同抗原。单克隆抗体22C4识别出BHT膜制剂中的三种多肽，其分子量分别为42、56和85 kDa。单克隆抗体22C4还识别出人心脏组织中的一种85 kDa成分和一种200 kDa成分。单克隆抗体D159对BHT膜中单一的82 kDa多肽具有特异性，并且还与人心脏中的两种高分子量成分（165和200 kDa）结合。当单克隆抗体D159和22C4都先用变形链球菌P-4细胞吸收时，随后对上述BHT膜成分的反应性受到抑制，这表明这些交叉反应性成分在变形链球菌P-4以及变形链球菌BHT微生物中都存在。竞争性结合分析表明，单克隆抗体D159和单克隆抗体22C4都与肌球蛋白结合，这表明变形链球菌BHT膜、人心脏组织和肌球蛋白至少共享一个免疫决定簇。这表明肌球蛋白可能是人心脏中的交叉反应性组织成分。
