Ayakawa G Y, Siegel J L, Crowley P J, Bleiweis A S
Infect Immun. 1985 May;48(2):280-6. doi: 10.1128/iai.48.2.280-286.1985.
Cell membranes of Streptococcus mutans BHT serotype b were prepared after glass bead disruption or mutanolysin digestion of whole cells. Immunoblot analyses of BHT membrane extracts revealed major polypeptides of 42,000, 46,000, 62,000, and 82,000 daltons, as well as several minor bands, to be reactive with rabbit anti-human heart immunoglobulins. Heart cross-reactive antigens have been reported in the cell walls and culture fluids of several S. mutans serotypes. This represents the first report of cell membrane-localized heart cross-reactive antigens in this oral pathogen. Positive enzyme-linked immunosorbent assay and immunoblot reactions were also obtained with heart tissue antigen and anti-BHT sera, indicating mutual cross-reactivity. The major cross-reactive component detected by immunoblotting of human heart extracts was a 69,000-dalton polypeptide.
变形链球菌BHT b血清型的细胞膜是在对全细胞进行玻璃珠破碎或变溶菌素消化后制备的。对BHT膜提取物的免疫印迹分析显示，42000、46000、62000和82000道尔顿的主要多肽以及几条次要条带与兔抗人心脏免疫球蛋白发生反应。在几种变形链球菌血清型的细胞壁和培养液中已报道有心脏交叉反应性抗原。这是关于这种口腔病原体中细胞膜定位的心脏交叉反应性抗原的首次报道。用心脏组织抗原和抗BHT血清也获得了阳性酶联免疫吸附测定和免疫印迹反应，表明存在相互交叉反应性。通过对人心脏提取物进行免疫印迹检测到的主要交叉反应成分是一种69000道尔顿的多肽。
