Suriano Francesca, Santini Daniele, Perrone Giuseppe, Amato Michela, Vincenzi Bruno, Tonini Giuseppe, Muda Andrea, Boggia Sara, Buscarini Maurizio, Pantano Francesco
J Exp Clin Cancer Res. 2013 Nov 5;32(1):87. doi: 10.1186/1756-9966-32-87.
To evaluate the prognostic role of TAMs in patients affected by non-muscle invasive bladder cancer (NMIBC), undergone Trans Urethral Resection of Bladder (TURB) and Bacillus Calmette-Guerin (BCG) therapy.
Data from 40 patients (36 men, 4 women), mean age 69 years (40-83 years), treated for NMIBC with TURB and BCG instillation were collected. Two different groups were considered: group with and group without bladder cancer recurrence. Correlations between immunofluorescence measured Mtot, M1 and M2 infiltration and clinicopathological parameters were evaluated using Spearman and Mann-Whitney methods. The recurrence-free survival rate was calculated using the Kaplan-Meier method.
CD68 positive cells (Mtot) were observed in all specimens tested. High Mtot, M1 and M2 infiltration was observed in patients with disease recurrence, even before endovescical BCG instillation. Significant value for M2 infiltration (p = 0,042) was found calculating significativity between two group medians before BCG therapy. p = 0,072 and p = 0,180 were observed correlating median of Mtot and M1 between two groups of patients respectively. Values of p = 0,44, p = 0,23 and p = 0,64 from correlation between DFS and Mtot, M1 and M2 median in patients before endovescical BCG instillation, were calculated respectively. Comparing DFS and Mtot, M1 and M2 median in patients group after endovescical BCG instillation significant values were obtained (p = 0,020; p = 0,02; and p = 0,029 respectively).
M2 tumor infiltration could be a prognostic value of recurrence in patients with NMIBC.
评估肿瘤相关巨噬细胞（TAMs）在接受经尿道膀胱肿瘤切除术（TURB）和卡介苗（BCG）治疗的非肌层浸润性膀胱癌（NMIBC）患者中的预后作用。
收集40例（36例男性，4例女性）平均年龄69岁（40 - 83岁）接受TURB和BCG灌注治疗NMIBC患者的数据。分为两个不同组：有膀胱癌复发组和无膀胱癌复发组。采用Spearman和Mann-Whitney方法评估免疫荧光测量的Mtot、M1和M2浸润与临床病理参数之间的相关性。使用Kaplan-Meier方法计算无复发生存率。
在所有检测标本中均观察到CD68阳性细胞（Mtot）。在疾病复发患者中观察到高Mtot、M1和M2浸润，甚至在膀胱内BCG灌注前。在BCG治疗前计算两组中位数之间的显著性时，发现M2浸润具有显著意义（p = 0.042）。分别观察到两组患者Mtot和M1中位数之间的相关性p值为0.072和0.180。在膀胱内BCG灌注前患者中，DFS与Mtot、M1和M2中位数之间的相关性计算出的p值分别为0.44、0.23和0.64。比较膀胱内BCG灌注后患者组的DFS与Mtot、M1和M2中位数，获得了显著值（分别为p = 0.020；p = 0.02；和p = 0.029）。
M2肿瘤浸润可能是NMIBC患者复发的预后指标。
