Bulut Yasemin, Bahcecioglu Ibrahim Halil, Aygun Cem, Oner Pinar Demirel, Ozercan Ibrahim, Demirdag Kutbeddin
Faculty of Medicine, Firat University, Elazig, Turkey.
J Infect Dev Ctries. 2014 Jan 15;8(1):74-8. doi: 10.3855/jidc.3910.
Hepatitis delta virus (HDV) is a serious cause of liver-related mortality in patients infected with hepatitis B virus (HBV). Determination of genotypes of HDV and phylogenetic analysis are important for better understanding the pathogenesis of the liver diseases associated with HBV infection. The aim of this study was to determine the genotype or genotypes of HDV among chronically infected patients with HBV in eastern Turkey.
A group of 113 patients infected with HBV and HDV were included in this study. The samples taken from the patients were analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR) and restriction enzyme cleavage.
According to the results of the restriction enzyme analysis, all of the RT-PCR products were determined to be HDV genotype I. Furthermore, for phylogenetic analysis and genotyping, 40 of HDV RT-PCR positive products were sequenced. Phylogenetic analysis of the sequences showed that all of the samples were infected with HDV genotype I. In addition, the results of the alignment analysis showed that the sequences of clinical samples were 82%-95% similar.
These results indicate that high genetic diversity of the virus is possible in endemic areas such as Turkey.
丁型肝炎病毒（HDV）是感染乙型肝炎病毒（HBV）患者肝脏相关死亡的一个严重原因。确定HDV基因型及进行系统发育分析对于更好地理解与HBV感染相关的肝脏疾病发病机制很重要。本研究的目的是确定土耳其东部慢性感染HBV患者中HDV的基因型。
本研究纳入了一组113例感染HBV和HDV的患者。从患者采集的样本通过逆转录聚合酶链反应（RT-PCR）和限制性酶切进行分析。
根据限制性酶切分析结果，所有RT-PCR产物均被确定为HDV基因型I。此外，为进行系统发育分析和基因分型，对40个HDV RT-PCR阳性产物进行了测序。序列的系统发育分析表明，所有样本均感染了HDV基因型I。此外，比对分析结果显示临床样本序列的相似性为82%-95%。
这些结果表明，在土耳其等流行地区，该病毒可能具有高度的遗传多样性。
Zotero 插件