Mohebbi Seyed Reza, Zali Narges, Derakhshan Faramarz, Tahami Ali, Mashayekhi Reza, Amini-Bavil-Olyaee Samad, Zali Mohammad Reza
The Research Center for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Med Virol. 2008 Dec;80(12):2092-9. doi: 10.1002/jmv.21326.
To identify hepatitis delta virus (HDV) genetic variability and its circulating genotypes amongst infected Iranian patients, 25 patients with positive anti-HDV status from different parts of Iran were enrolled in this cross-sectional study. A portion of the HDV delta antigen was amplified, sequenced, and subjected to molecular and phylogenetic analysis. Clinical features and virological markers were evaluated. HDV RNA could be detected in 88% of anti-HDV positive cases (22 patients) with chronic hepatitis B virus (HBV) infection and liver cirrhosis. Phylogenetic analysis revealed that all Iranian patients were infected by genotype I (clade 1) of HDV, supported by a high bootstrap value (100%, 1,000 replicates). All HDV-positive patients were coinfected with genotype D1 of HBV. No significant association was determined between demographic, clinical, and virological variables in the population studied. In conclusion, the present molecular epidemiology survey reveals that clade 1 of HDV is predominant among coinfected HBV patients in Iran.
为了确定伊朗感染患者中丁型肝炎病毒（HDV）的基因变异性及其流行基因型，本横断面研究纳入了25名来自伊朗不同地区抗HDV状态呈阳性的患者。对HDVδ抗原的一部分进行扩增、测序，并进行分子和系统发育分析。评估临床特征和病毒学标志物。在88%（22例患者）的慢性乙型肝炎病毒（HBV）感染和肝硬化的抗HDV阳性病例中可检测到HDV RNA。系统发育分析显示，所有伊朗患者均感染HDV基因型I（分支1），自展值较高（100%，1000次重复）支持这一结果。所有HDV阳性患者均合并感染HBV D1基因型。在所研究的人群中，人口统计学、临床和病毒学变量之间未确定显著关联。总之，目前的分子流行病学调查显示，HDV分支1在伊朗合并感染HBV的患者中占主导地位。
