Fan Xiao-mei, Li Kui-xiu, Niu Shu-huai, Fang Zhao-hui, Liu Hong
Department of Gynecological Oncology, Fourth Hospital, Hebei Medical University, Shijiazhuang 050011, China.
Department of Gynecological Oncology, Fourth Hospital, Hebei Medical University, Shijiazhuang 050011, China. Email:
Zhonghua Yi Xue Za Zhi. 2013 Nov 19;93(43):3454-6.
To explore the correlation of XRCC1 Arg194Trp Arg399Gln SNPs with the risks and clinicopathological factors of cervical cancer.
Polymorphisms Arg194Trp, Arg399Gln of XRCC1 gene in 253 cervical cancer patients and 350 healthy controls were analyzed by mismatch amplification polymerase chain reaction (MAMA-PCR).
Compared with the Arg/Arg genotypes, the Trp/Trp genotypes could increase the risk of cervical cancer (P = 0.000) . Arg/Arg was a protection factor with an odds ratio of 0.116 (95%CI = 0.043-0.316) . The frequency of Arg and Gln allele among cervical cancer and healthy controls were 56.81%, 43.19% and 64.86%, 35.14%. Compared with the Arg genotypes, the Gln genotypes could increase the risk of cervical cancer (P < 0.05) . Compared with the Arg/Arg genotypes, the Gln/Gln genotypes could increase the risk of cervical cancer (P < 0.01) . Arg/Arg was a protection factor with an odds ratio of 0.188 (95%CI = 0.089-0.398) . Using haplotypes 194Trp-399Gln as a reference, women carrying 194Arg-399Gln and 194Arg-399Arg halpotypes had a significantly decreased risk for cervical carcinoma. The adjusted OR and 95%CI were 0.613 [0.446, 0.842] and 0.635 [0.449, 0.899]. The frequencies of Arg/Arg, Arg/Gln, Gln/Gln genotype had significant relationship with clinical stage and histological grade. Tumor diameter, patient age and neoplastic form had no relationship.
Compared with the Arg/Arg genotypes, the Trp/Trp and Gln/ Gln genotypes could increase the risk of cervical cancer. The Trp or Gln genotypes risk increases. Arg may be a protection factor. The frequencies of Arg399Gln SNP have a correlation with stage and histological grade.
探讨XRCC1基因Arg194Trp、Arg399Gln单核苷酸多态性（SNP）与宫颈癌发病风险及临床病理因素的相关性。
采用错配扩增聚合酶链反应（MAMA-PCR）技术，对253例宫颈癌患者和350例健康对照者的XRCC1基因Arg194Trp、Arg399Gln多态性进行分析。
与Arg/Arg基因型相比，Trp/Trp基因型可增加宫颈癌发病风险（P = 0.000）。Arg/Arg是保护因素，比值比为0.116（95%可信区间=0.043 - 0.316）。宫颈癌组和健康对照组中Arg和Gln等位基因频率分别为56.81%、43.19%和64.86%、35.14%。与Arg基因型相比，Gln基因型可增加宫颈癌发病风险（P < 0.05）。与Arg/Arg基因型相比，Gln/Gln基因型可增加宫颈癌发病风险（P < 0.01）。Arg/Arg是保护因素，比值比为0.188（95%可信区间=0.089 - 0.398）。以单倍型194Trp - 399Gln为参照，携带194Arg - 399Gln和194Arg - 399Arg单倍型的女性患宫颈癌风险显著降低。校正后的比值比及95%可信区间分别为0.613[0.446, 0.842]和0.635[0.449, 0.899]。Arg/Arg、Arg/Gln、Gln/Gln基因型频率与临床分期和组织学分级有显著相关性。肿瘤直径、患者年龄和肿瘤形态无相关性。
与Arg/Arg基因型相比，Trp/Trp和Gln/Gln基因型可增加宫颈癌发病风险。Trp或Gln基因型风险增加。Arg可能是保护因素。Arg399Gln SNP频率与临床分期和组织学分级相关。
