Centre de recherche en photobiophysique, Université du Québec à Trois-Rivières, C.P. 500, Trois-Rivières, (Québec), Canada, G9A 5H7.
Photosynth Res. 1989 Nov;22(2):131-6. doi: 10.1007/BF00035443.
Oxygen evolution in Dunaliella tertiolecta under flashing light was measured with a bare electrode, at a 10 Hz frquency. The 'sigma coefficients' of the oxygen evolution recurrence law (Thibault (1978) J Theor Biol 73, 271) were determined using groups of nine consecutive points. The S-state transition probabilities were computed from the sigma coefficients and plotted as a function of the flash number of the first of the points used. Low standard deviations over the sigma coefficients resulted from the use of our system (Meunier & Popovic (1988) Rev Sci Instr 59, 486). We observed a linear lowering of the miss probability with time, and a linear rise of the single-hit probability with the same absolute value of the slope. The hypothesis that the slopes were zero was statistically tested and was rejected with a 99.9% confidence interval. Our work demonstrates that, to be accurate, an oxygen evolution model has to take the variations in the properties of S-states into account.
在 10 Hz 的频率下,使用裸电极测量了闪光条件下杜氏盐藻中的氧气释放。利用九组连续点确定了氧气释放重现律的“σ系数”(Thibault(1978)J Theor Biol 73, 271)。从σ系数计算 S 态跃迁概率,并将其绘制成所使用的第一点的闪光次数的函数。我们系统的使用(Meunier 和 Popovic(1988)Rev Sci Instr 59, 486)产生了 σ系数的低标准偏差。我们观察到错过概率随时间呈线性降低,而单次命中概率则以相同的斜率绝对值呈线性增加。统计检验了斜率为零的假设,并以 99.9%的置信区间拒绝了该假设。我们的工作表明,为了准确,氧气释放模型必须考虑 S 态特性的变化。