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用杜氏盐藻的氧释放测量的闪光次数与 miss 和单 hit S 态概率的线性变化的证据。

Evidence for a linear variation of the miss and single hit S-state probabilities with the flash number, measured by oxygen evolution in Dunaliella tertiolecta.

机构信息

Centre de recherche en photobiophysique, Université du Québec à Trois-Rivières, C.P. 500, Trois-Rivières, (Québec), Canada, G9A 5H7.

出版信息

Photosynth Res. 1989 Nov;22(2):131-6. doi: 10.1007/BF00035443.

DOI:10.1007/BF00035443
PMID:24424745
Abstract

Oxygen evolution in Dunaliella tertiolecta under flashing light was measured with a bare electrode, at a 10 Hz frquency. The 'sigma coefficients' of the oxygen evolution recurrence law (Thibault (1978) J Theor Biol 73, 271) were determined using groups of nine consecutive points. The S-state transition probabilities were computed from the sigma coefficients and plotted as a function of the flash number of the first of the points used. Low standard deviations over the sigma coefficients resulted from the use of our system (Meunier & Popovic (1988) Rev Sci Instr 59, 486). We observed a linear lowering of the miss probability with time, and a linear rise of the single-hit probability with the same absolute value of the slope. The hypothesis that the slopes were zero was statistically tested and was rejected with a 99.9% confidence interval. Our work demonstrates that, to be accurate, an oxygen evolution model has to take the variations in the properties of S-states into account.

摘要

在 10 Hz 的频率下,使用裸电极测量了闪光条件下杜氏盐藻中的氧气释放。利用九组连续点确定了氧气释放重现律的“σ系数”(Thibault(1978)J Theor Biol 73, 271)。从σ系数计算 S 态跃迁概率,并将其绘制成所使用的第一点的闪光次数的函数。我们系统的使用(Meunier 和 Popovic(1988)Rev Sci Instr 59, 486)产生了 σ系数的低标准偏差。我们观察到错过概率随时间呈线性降低,而单次命中概率则以相同的斜率绝对值呈线性增加。统计检验了斜率为零的假设,并以 99.9%的置信区间拒绝了该假设。我们的工作表明,为了准确,氧气释放模型必须考虑 S 态特性的变化。

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Evidence for a linear variation of the miss and single hit S-state probabilities with the flash number, measured by oxygen evolution in Dunaliella tertiolecta.用杜氏盐藻的氧释放测量的闪光次数与 miss 和单 hit S 态概率的线性变化的证据。
Photosynth Res. 1989 Nov;22(2):131-6. doi: 10.1007/BF00035443.
2
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引用本文的文献

1
Control of misses in oxygen evolution by the oxido-reduction state of plastoquinone in Dunaliella tertiolecta.通过中间态电子载体在杜氏盐藻中控制氧释放的错误。
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2
Improvement of four sigma analysis for the investigation of oxygen evolution by Photosystem II.四西格玛分析在研究光合系统 II 氧气产生中的应用改进。
Photosynth Res. 1991 Aug;29(2):113-5. doi: 10.1007/BF00035382.
3
On the proper use of sigma analysis in the study of flash-induced oxygen evolution.关于在闪光诱导氧气释放研究中正确使用西格玛分析的讨论。

本文引用的文献

1
Optimization of the bare platinum electrode as an oxygen measurement system in photosynthesis.优化裸铂电极作为光合作用中氧测量系统。
Photosynth Res. 1988 Mar;15(3):271-9. doi: 10.1007/BF00047358.
2
Light intensity saturation properties of O2 yields in a sequence of flashes in Chlorella.小球藻中一系列闪光下氧气产量的光强饱和特性
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Photosynth Res. 1991 Jan;27(1):15-8. doi: 10.1007/BF00029972.
4
Oxygen evolution by Photosystem II: The contribution of backward transitions to the anomalous behaviour of double-hits revealed by a new analysis method.光系统 II 的氧气生成:通过新的分析方法揭示的双击中异常行为的反向跃迁贡献。
Photosynth Res. 1993 May;36(2):111-8. doi: 10.1007/BF00016276.
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6
Flash-induced oxygen evolution in photosynthesis: simple solution for the extended S-state model that includes misses, double-hits, inactivation, and backward-transitions.光合作用中闪光诱导的氧气释放:扩展S态模型的简单解决方案,该模型包括失误、双击、失活和逆向转变。
Biophys J. 2005 Jan;88(1):412-21. doi: 10.1529/biophysj.104.050898. Epub 2004 Oct 8.
利用不同持续时间的激发闪光对光系统II电荷分离和氧气释放中的双周转进行研究。
Biochim Biophys Acta. 1981 Mar 12;635(1):38-52. doi: 10.1016/0005-2728(81)90005-0.
4
Cooperation of charges in photosynthetic O2 evolution-I. A linear four step mechanism.光合作用中氧气释放过程中电荷的协同作用 - I. 线性四步机制。
Photochem Photobiol. 1970 Jun;11(6):457-75. doi: 10.1111/j.1751-1097.1970.tb06017.x.
5
A kinetic analysis of the oxidizing and reducing sides of the O2-evolving system of photosynthesis.光合作用中氧气释放系统氧化侧和还原侧的动力学分析。
Biochim Biophys Acta. 1973 Jul 26;314(1):28-41. doi: 10.1016/0005-2728(73)90061-3.
6
Electron transfer in the water-oxidizing complex of Photosystem II.光系统II水氧化复合物中的电子转移
J Bioenerg Biomembr. 1987 Apr;19(2):125-42. doi: 10.1007/BF00762721.
7
Matrix analysis of the oxygen evolving system of photosynthesis.光合作用放氧系统的矩阵分析
J Theor Biol. 1976 Mar;57(1):171-85. doi: 10.1016/s0022-5193(76)80011-2.
8
Anomalies in the kinetics of photosynthetic oxygen emission in sequences of flashes revealed by matrix analysis. Effects of carbonyl cyanide m-chlorophenylhydrazone and variation in time parameters.通过矩阵分析揭示的闪光序列中光合氧释放动力学异常。羰基氰化物间氯苯腙的影响及时间参数变化。
Biochim Biophys Acta. 1976 Jun 8;430(3):501-16. doi: 10.1016/0005-2728(76)90026-8.
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Dependence of the deactivation reactions of photosystem II on the redox state of plastoquinone pool A varied under anaerobic conditions; Equilibria on the acceptor side of photosystem II.在厌氧条件下,光系统II失活反应对质体醌库A氧化还原状态的依赖性发生变化;光系统II受体侧的平衡。
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10
A new attempt to study the oxygen evolving system of photosynthesis: determination of transition probabilities of a state i.研究光合作用放氧系统的新尝试:状态i跃迁概率的测定
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