Suppr超能文献

洋葱发育保卫细胞中纤维素微纤丝取向和细胞形态形成:微管和离子积累的作用。

Cellulose microfibril orientation and cell shaping in developing guard cells of Allium: The role of microtubules and ion accumulation.

机构信息

Department of Cellular and Comparative Biology, State University of New York, 11794, Stony Brook, NY, USA.

出版信息

Planta. 1976 Jan;132(1):71-93. doi: 10.1007/BF00390333.

Abstract

The role of microtubules and ions in cell shaping was investigated in differentiating guard cells of Allium using light and electron microscopy and cytochemistry. Microtubules appear soon after cytokinesis in a discrete zone close to the plasmalemma adjacent to the common wall between guard cells. The microtubules fan out from this zone, which corresponds to the future pore site, towards the other sides of the cell. Soon new cellulose microfibrils are deposited on the wall adjacent to the microtubules and oriented parallel to them. As the wall thickens, the shape of the cell shifts from cylindrical to kidney-like. Studies with polarized light show that guard cells gradually assume a birefringence pattern during development characteristic of wall microfibrils radiating away from the pore site. Retardation increases from 10 Å when cells just begin to take shape, to 80-100 Å at maturity. Both microfibril and microtubule orientation remain constant during development. Observations on aberrant cells including those produced under the influence of drugs such as colchicine, which leads to loss of microtubules, abnormal wall thickenings and disruption of wall birefringence, further support the role of microtubules in cell shaping through their function in the localization of wall deposition and the orientation of cellulose microfibrils in the new wall layer. Potassium first appears in guard mother cells before division and rapidly accumulates afterwards during cell shaping, as judged by the cobaltinitrite reaction. Some chloride and perhaps organic acid anions also accumulate. Thus, these ions, which are known to play a role in the function of mature guard cells, also seem to be important in the early growth and shaping of these cells.

摘要

使用光镜和电镜细胞化学技术研究了微管和离子在洋葱保卫细胞分化中的作用。在胞质分裂后不久,微管出现在靠近相邻保卫细胞共同细胞壁的质膜附近的一个离散区域。微管从这个区域扇形展开,对应未来的孔位点,朝向细胞的另一侧。不久,新的纤维素微纤维在与微管相邻的壁上沉积,并与它们平行排列。随着细胞壁变厚,细胞的形状从圆柱形变为肾形。偏振光研究表明,保卫细胞在发育过程中逐渐呈现出特征性的壁微纤维从孔位点发散的双折射模式。当细胞刚刚开始形成时,延迟增加到 10Å,成熟时增加到 80-100Å。在发育过程中,微纤维和微管的取向保持不变。对异常细胞的观察,包括在秋水仙素等药物影响下产生的异常细胞,这些药物导致微管丧失、细胞壁异常增厚和细胞壁双折射破坏,进一步支持微管在细胞成形中的作用,通过其在壁沉积的定位和新壁层中纤维素微纤维的取向的功能。钾在分裂前出现在保卫母细胞中,在细胞成形过程中迅速积累,这可以通过钴亚硝酸反应来判断。一些氯离子,也许还有有机酸阴离子也会积累。因此,这些在成熟保卫细胞功能中起作用的离子,在这些细胞的早期生长和成形中似乎也很重要。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验