嗜热脂肪芽孢杆菌 G-82 细胞生长和普鲁兰酶生产受碳源和溶解氧水平的影响。

Effect of carbon source and dissolved oxygen level on cell growth and pullulanase production byBacillus stearothermophilus G-82.

机构信息

Department of Enzyme Biosynthesis, Institute of Microbiology, Acad. G. Bonchev str. B1.26, 1113, Sofia, Bulgaria.

出版信息

World J Microbiol Biotechnol. 1992 Jan;8(1):21-3. doi: 10.1007/BF01200678.

DOI:10.1007/BF01200678

Abstract

Cell growth and extracellular pullulanase production ofBacillus stearothermophilus G-82 were investigated in batch culture using a defined medium with glucose, maltose, pullulan or amylopectin as carbon source. Maximum enzyme activity was with pullulan or amylopectin. Cell growth in batch culture was better under oxygen unlimited conditions, while higher total and specific enzyme activities, using pullulan or amylopectin, were obtained in oxygen-limited conditions. Enzyme accumulation took place in the late growth phase. The highest enzyme production of 300 U/I was reached when pullulan was used as carbon source in conditions of oxygen limitation.

摘要

采用含有葡萄糖、麦芽糖、普鲁兰或支链淀粉的限定培养基，研究了嗜热脂肪芽孢杆菌 G-82 的细胞生长和胞外普鲁兰酶的生产。最大酶活与普鲁兰或支链淀粉有关。在供氧不受限制的条件下，分批培养中的细胞生长更好，而在供氧有限的条件下，使用普鲁兰或支链淀粉可获得更高的总酶和比酶活。酶的积累发生在生长后期。当使用普鲁兰作为碳源，并在供氧有限的条件下，可达到 300 U/I 的最高酶产量。