高产漆酶的佛罗里达侧耳突变体。

High laccase producing mutants ofPleurotus florida.

机构信息

Department of Microbiology, Punjab Agricultural University, 141 004, Ludhiana, India.

出版信息

World J Microbiol Biotechnol. 1992 Jan;8(1):39-41. doi: 10.1007/BF01200681.

DOI:10.1007/BF01200681

Abstract

Pleurotus florida produced high amounts of laccase (4.60 U/ml) in malt extract broth after 12 days' growth under stationary conditions. The production of laccase was semi-constitutive. Hyperlaccase mutants ofP. florida were obtained through mutagenesis of mycelial protoplasts usingN-methyl-N'-nitro-N-nitrosoguanidine (50 μg/ml) for 2 min. Three hyperlaccase mutants were selected showing growth and enzyme production responses similar to the parent.

摘要

佛罗里达侧耳在静置条件下培养 12 天后，在麦芽提取物培养基中产生了大量漆酶（4.60 U/ml）。漆酶的产生是半组成型的。通过使用 N-甲基-N'-硝基-N-亚硝基胍（50 μg/ml）对菌丝体原生质体进行诱变，获得了佛罗里达侧耳的高漆酶突变体。选择了三个高漆酶突变体，它们的生长和产酶反应与亲本相似。