Division of Biology, National Yang-Ming Medical College, Taipei, Taiwan, Republic of China.
World J Microbiol Biotechnol. 1992 Mar;8(2):141-6. doi: 10.1007/BF01195834.
Eighteen strains of xylariaceous fungi have been screened for higher activities of cellulolytic enzymes,Trichoderma reesei QM 9414 was also examined for comparison. Strains ofXylaria anisopleura andX. regalis had higher endocellulase (CMCase) and exocellulase (Avicelase) activities after 2 weeks' incubation.Hypoxylon stygium produced the highest activity of β-glucosidase 3 days after inoculation. The optimum pH for these cellulolytic enzymes was approx. 5.0 and the optimum temperatures ranged from 37 to 50°C. A mixed culture process usingT. reesei QM 9414 andH. stygium was developed to obtain enhanced synthesis of cellulase. β-Glucosidase activities in the mixed culture increased within 48h whenH. stygium was introduced after 24h.
已经筛选了 18 株木霉真菌菌株，以寻找具有更高纤维素酶活性的菌株，同时也对里氏木霉 QM 9414 进行了检测作为比较。在培养 2 周后，平鳞侧耳（Xylaria anisopleura）和蜡伞（X. regalis）菌株的内切纤维素酶（CMCase）和外切纤维素酶（Avicelase）活性更高。在接种后 3 天，暗皮壳菌（Hypoxylon stygium）产生的β-葡萄糖苷酶活性最高。这些纤维素酶的最适 pH 值约为 5.0，最适温度范围为 37 至 50°C。开发了一种使用里氏木霉 QM 9414 和暗皮壳菌的混合培养工艺，以获得增强的纤维素酶合成。当在接种后 24 小时后引入暗皮壳菌时，混合培养物中的β-葡萄糖苷酶活性在 48 小时内增加。
