嗜热枝孢霉产生纤维素酶的影响因素。
Factors influencing the production of cellulases by Sporotrichum thermophile.
作者信息
Coutts A D, Smith R E
出版信息
Appl Environ Microbiol. 1976 Jun;31(6):819-25. doi: 10.1128/aem.31.6.819-825.1976.
Abstract
Cellulase production and growth of a strain of Sporotrichum thermophile were studied by using a mineral salts medium supplemented with yeast extract and insoluble cellulose. The effects of cultural conditions, such as pH, nitrogen source, substrate concentration, and temperature, were examined. Maximum production of C1 and CX cellulases occurred at 45 C in 2 to 4 days, in the presence of 1% Solka/Floc as substrate, when NaNO3 or urea used as sources of nitrogen. Under these conditions, cellulolytic activity of culture filtrates appeared to be similar to that reported for Trichoderma viride grown in a favorable environment. However, comparable yields of cellulase were produced by S. thermophile in less than one-quarter the time required by mesophilic fungi.
摘要
通过使用添加了酵母提取物和不溶性纤维素的矿物盐培养基，研究了嗜热侧孢霉菌株的纤维素酶产生和生长情况。考察了培养条件如pH、氮源、底物浓度和温度的影响。当以1%的索尔卡纤维为底物、使用硝酸钠或尿素作为氮源时，在2至4天内于45℃下C1和CX纤维素酶的产量最高。在这些条件下，培养滤液的纤维素分解活性似乎与在有利环境中生长的绿色木霉所报道的活性相似。然而，嗜热侧孢霉产生的纤维素酶产量相当，但所需时间不到嗜温真菌所需时间的四分之一。
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