在大肠杆菌中克隆具有启动子活性的须癣毛癣菌 DNA 序列。
Molecular cloning ofTrichophyton mentagrophytes DNA sequences with promoter activity inEscherichia coli.
机构信息
Departament de Microbiologia, Facultat de Ciències Biològiques, Universitat de València, Calle Dr. Moliner 50, 46100, Valencia, Spain.
出版信息
World J Microbiol Biotechnol. 1992 Mar;8(2):196-8. doi: 10.1007/BF01195847.
A promoter probe library from the dermatophyte fungusTrichophyton mentagrophytes has been constructed in the pVB32 plasmid vector. Using this library, a set ofT. mentagrophytes DNA sequences with promoter activity inEscherichia coli has been cloned. The size and the resistance phenotype conferred by these DNA fragments varied. Southern blot analysis confirmed that they were derived fromT. mentagrophytes genomic DNA.
已在 pVB32 质粒载体中构建了来自亲表皮真菌须癣毛癣菌的启动子探针文库。利用该文库，在大肠杆菌中克隆了一组具有启动子活性的须癣毛癣菌 DNA 序列。这些 DNA 片段的大小和赋予的抗性表型不同。Southern blot 分析证实它们源自须癣毛癣菌的基因组 DNA。
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