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须癣毛癣菌向潮霉素B耐药性的转变。

Transformation of the dermatophyte Trichophyton mentagrophytes to hygromycin B resistance.

作者信息

Gonzalez R, Ferrer S, Buesa J, Ramon D

机构信息

Departament de Microbiologia, Facultat de Biologia, Universitat de Valéncia, Spain.

出版信息

Infect Immun. 1989 Sep;57(9):2923-5. doi: 10.1128/iai.57.9.2923-2925.1989.

DOI:10.1128/iai.57.9.2923-2925.1989
PMID:2759709
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC313551/
Abstract

A transformation system for the ringworm-producing dermatophyte Trichophyton mentagrophytes has been developed. The system employs the plasmid pHIS, which contains a bacterial hygromycin B phosphotransferase gene linked to Cochliobolus heterostrophus regulatory sequences (B. G. Turgeon, R. C. Garber, and O. C. Yoder, Mol. Cell. Biol. 7:3297-3305, 1987). This plasmid confers hygromycin B resistance to T. mentagrophytes. The DNA was stably integrated into the fungal genome, and the number and sites of integrations varied among transformants. Transformant clones were capable of infecting guinea pigs. This system opens the way for the molecular genetic analysis of the interaction of T. mentagrophytes with epithelial animal tissues.

摘要

已开发出一种用于产生癣的皮肤癣菌须癣毛癣菌的转化系统。该系统使用质粒pHIS，它含有一个与玉米小斑病菌调控序列相连的细菌潮霉素B磷酸转移酶基因（B.G. 特金、R.C. 加伯和O.C. 约德，《分子与细胞生物学》7:3297 - 3305，1987年）。该质粒赋予须癣毛癣菌潮霉素B抗性。DNA稳定整合到真菌基因组中，整合的数量和位点在转化体之间有所不同。转化体克隆能够感染豚鼠。该系统为须癣毛癣菌与动物上皮组织相互作用的分子遗传分析开辟了道路。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b413/313551/425ae01812b7/iai00069-0346-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b413/313551/c621b7c9a2c4/iai00069-0346-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b413/313551/425ae01812b7/iai00069-0346-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b413/313551/c621b7c9a2c4/iai00069-0346-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b413/313551/425ae01812b7/iai00069-0346-b.jpg

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