Cellulases ofVibrio agar-liquefaciens isolated from sea mud.

Vibrio agar-liquefaciens produced the component enzymes of a cellulase complex (exoglucanase, endoglucanase and β-glucosidase). Whilst complete cellulase activity occurred in three media, each containing carboxymethylcellulose as the sole carbon source, different activities of the individual enzymes resulted. The enzymes showed a temporal sequence in their activity and differed in pH and temperature optima. NaCl enhanced the enzyme activities to varying degrees at different substrate concentrations.