Levin L, Forchiassin F
Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
Rev Argent Microbiol. 1995 Jan-Mar;27(1):11-20.
Trametes trogii was grown in a liquid synthetic medium containing different carbon and nitrogen sources. Enzymatic activities of cellulases (endoglucanase, exoglucanase and beta-glucosidase) were measured in culture supernatants. Organic nitrogen sources were the most favourable for growth and cellulase production. Increasing nitrogen concentrations also increased cellulase production. Among carbon sources, crystalline cellulose, cellobiose and a mixture of carboxymethylcellulose and cellobiose induced maximal endoglucanase production. The optimal concentration of the carbon source was 10 g/l.
毛栓菌在含有不同碳源和氮源的液体合成培养基中生长。在培养上清液中测定纤维素酶(内切葡聚糖酶、外切葡聚糖酶和β-葡萄糖苷酶)的酶活性。有机氮源最有利于生长和纤维素酶的产生。增加氮浓度也会增加纤维素酶的产量。在碳源中,结晶纤维素、纤维二糖以及羧甲基纤维素和纤维二糖的混合物诱导产生最大量的内切葡聚糖酶。碳源的最佳浓度为10 g/l。