Department of Chemistry and Chemical Engineering, Beijing Forestry University, Beijing 100083, China.
Bioprocess Biosyst Eng. 2011 Mar;34(3):357-65. doi: 10.1007/s00449-010-0478-7. Epub 2010 Dec 10.
The cellulase production by Trichoderma viride, cultivated on different substrates, namely steam-pretreated Lespedeza, filter paper, microcrystalline cellulose (MCC) or carboxymethyl cellulose (CMC), was studied. Different cellulase systems were secreted when cultivated on different substrates. The cellulolytic enzyme from steam-pretreated Lespedeza medium performed the highest filter paper activity, exoglucanase and endoglucanase activities, while the highest β-glucosidase activity was obtained from the enzyme produced on filter paper medium. The hydrolytic potential of the enzymes produced from different media was evaluated on steam-pretreated Lespedeza. The cellulase from steam-pretreated Lespedeza was found to have the most efficient hydrolysis capability to this specific substrate. The molecular weights of the cellulases produced on steam-pretreated Lespedeza, filter paper and MCC media were 33, 37 and 40 kDa, respectively, and the cellulase from CMC medium had molecular weights of 20 and 43 kDa. The degree of polymerization, crystallinity index and micro structure scanned by the scanning electron microscopy of degraded steam-pretreated Lespedeza residues were also studied.
研究了在不同基质（蒸汽预处理胡枝子、滤纸、微晶纤维素（MCC）或羧甲基纤维素（CMC））上培养的绿色木霉产生的纤维素酶。在不同的基质上培养时，分泌了不同的纤维素酶系统。来自蒸汽预处理胡枝子培养基的纤维素酶对滤纸表现出最高的活性、外切葡聚糖酶和内切葡聚糖酶活性，而滤纸培养基中产生的β-葡萄糖苷酶活性最高。用不同培养基产生的酶对蒸汽预处理胡枝子进行了水解潜力评估。发现来自蒸汽预处理胡枝子的纤维素酶对这种特定底物具有最高的水解能力。在蒸汽预处理胡枝子、滤纸和 MCC 培养基上产生的纤维素酶的分子量分别为 33、37 和 40 kDa，而 CMC 培养基上的纤维素酶的分子量为 20 和 43 kDa。还研究了降解的蒸汽预处理胡枝子残余物的扫描电子显微镜下的聚合度、结晶度指数和微观结构。
