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从越南土壤中分离出的嗜热淀粉酶产生菌。

Thermophilic amylase-producing bacteria from Vietnamese soils.

机构信息

Food Industries Research Institute, Km 8 Nguyen Trai Road, Thanh xuan, Dong da, Hanoi, Vietnam.

出版信息

World J Microbiol Biotechnol. 1992 Sep;8(5):505-8. doi: 10.1007/BF01201949.

DOI:10.1007/BF01201949
PMID:24425565
Abstract

Of 25 bacterial isolates from Vietnamese soils, two were identified asBacillus stearothermophilus and one asThermoactinomyces thalpophilus, both thermophilic, amylase-producing bacteria. Amylase activity was highest in the presence of cassava starch as carbon source and (NH4)2HPO4 as nitrogen source. The strains exhibit a high amylase productivity within the first 5 to 7 h of cultivation at 55°C. The crude enzyme had optima of pH 6.5 and 70°C.

摘要

从越南土壤中分离的 25 株细菌中,有 2 株被鉴定为嗜热脂肪芽孢杆菌,1 株为嗜热放线菌,均为产淀粉酶的嗜热菌。在以木薯淀粉为碳源和(NH4)2HPO4为氮源的条件下,淀粉酶活性最高。在 55°C 下培养的前 5 至 7 小时内,菌株表现出较高的淀粉酶生产力。粗酶的最适 pH 值为 6.5,最适温度为 70°C。

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本文引用的文献

1
A photometric method for the determination of alpha-amylase in blood and urine, with use of the starch-iodine color.一种利用淀粉-碘显色法测定血液和尿液中α-淀粉酶的光度法。
J Biol Chem. 1949 May;179(1):53-9.
2
Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature.根据热变性温度确定脱氧核糖核酸的碱基组成
J Mol Biol. 1962 Jul;5:109-18. doi: 10.1016/s0022-2836(62)80066-7.