利用响应面法优化解脂耶氏酵母 NCIM 3472 转化 l-酪氨酸生成 l-多巴。
Optimization of Biotransformation of l-Tyrosine to l-DOPA by Yarrowia lipolytica-NCIM 3472 Using Response Surface Methodology.
机构信息
Department of Biotechnology, Shivaji University, Vidyanagar, Kolhapur, 416004 India.
Department of Microbiology, Shivaji University, Kolhapur, 416004 India.
出版信息
Indian J Microbiol. 2013 Jun;53(2):194-8. doi: 10.1007/s12088-012-0346-z. Epub 2013 Jan 11.
l-DOPA (3,4-dihydroxyphenyl-l-alanine) is the most widely used drug for treatment of Parkinson's disease. In this study Yarrowia lipolytica-NCIM 3472 biomass was used for transformation of l-tyrosine to l-DOPA. The process parameters were optimized using response surface methodology (RSM). The optimum values of the tested variables for the production of l-DOPA were: pH 7.31, temperature 42.9 °C, 2.31 g l(-1) cell mass and 1.488 g l(-1)l-tyrosine. The highest yield obtained with these optimum parameters along with recycling of the cells was 4.091 g l(-1). This optimization of process parameters using RSM resulted in 4.609-fold increase in the l-DOPA production. The statistical analysis showed that the model was significant. Also coefficient of determination (R(2)) was 0.9758, indicating a good agreement between the experimental and predicted values of l-DOPA production. The highest tyrosinase activity observed was 7,028 U mg(-1) tyrosine. l-DOPA production was confirmed by HPTLC and HPLC analysis. Thus, RSM approach effectively enhanced the potential of Y. lipolytica-NCIM 3472 as an alternative source to produce l-DOPA.
l-DOPA（3,4-二羟基苯丙氨酸）是治疗帕金森病最广泛使用的药物。在这项研究中，利用产朊假丝酵母 NCIM 3472 生物质将 l-酪氨酸转化为 l-DOPA。利用响应面法（RSM）对工艺参数进行了优化。用于生产 l-DOPA 的测试变量的最佳值为：pH 值 7.31、温度 42.9°C、细胞质量 2.31 g/L 和 l-酪氨酸 1.488 g/L。在这些最佳参数下，细胞回收的最高产量为 4.091 g/L。使用 RSM 对工艺参数进行优化，使 l-DOPA 的产量增加了 4.609 倍。统计分析表明该模型是显著的。决定系数（R²）也为 0.9758，表明 l-DOPA 产量的实验值与预测值吻合良好。观察到的最高酪氨酸酶活性为 7,028 U/mg 酪氨酸。通过 HPTLC 和 HPLC 分析证实了 l-DOPA 的生产。因此，RSM 方法有效地提高了产朊假丝酵母 NCIM 3472 作为生产 l-DOPA 的替代来源的潜力。
Zotero 插件