Surwase Shripad N, Jadhav Shekhar B, Phugare Swapnil S, Jadhav Jyoti P
Department of Microbiology, Shivaji University, Kolhapur, 416004, India.
Department of Biochemistry, Shivaji University, Kolhapur, 416004, India.
3 Biotech. 2013 Jun;3(3):187-194. doi: 10.1007/s13205-012-0082-4. Epub 2012 Aug 7.
Melanins are predominantly indolic polymers which are extensively synthesized in animals, plants and microorganisms. It has wide applications in cosmetics, agriculture and medicine. In the present study, optimization of process parameters influencing melanin production was attempted using the response surface methodology (RSM) from Brevundimonas sp. SGJ. A Plackett-Burman design was used for screening of critical components, while further optimization was carried out using the Box-Behnken design. The optimum conditions observed were pH 5.31, tryptone 1.440 g l, L-tyrosine 1.872 g l and CuSO 0.0366 g l. Statistical analysis revealed that the model is significant with model F value 29.03 and R value 0.9667. The optimization of process parameters using RSM resulted in a 3.05-fold increase in the yield of melanin. The intermittent addition of L-tyrosine enhanced the melanin yield to 6.811 g l. The highest tyrosinase activity observed was 2,471 U mg at the 18th hour of the incubation period with dry cell weight of 0.711 g l. The melanin production was confirmed by UV-Visible spectroscopy, FTIR and EPR analysis. Thus, Brevundimonas sp. SGJ has the potential to be a new source for the production of melanin.
黑色素主要是吲哚聚合物，在动物、植物和微生物中广泛合成。它在化妆品、农业和医学领域有广泛应用。在本研究中，尝试使用来自短波单胞菌属SGJ的响应面法（RSM）优化影响黑色素产生的工艺参数。采用Plackett-Burman设计筛选关键成分，同时使用Box-Behnken设计进行进一步优化。观察到的最佳条件为pH 5.31、胰蛋白胨1.440 g/l、L-酪氨酸1.872 g/l和硫酸铜0.0366 g/l。统计分析表明，该模型显著，模型F值为29.03，R值为0.9667。使用RSM对工艺参数进行优化后，黑色素产量提高了3.05倍。间歇添加L-酪氨酸可使黑色素产量提高到6.811 g/l。在培养期第18小时，干细胞重量为0.711 g/l时，观察到的最高酪氨酸酶活性为2471 U/mg。通过紫外可见光谱、傅里叶变换红外光谱和电子顺磁共振分析确认了黑色素的产生。因此，短波单胞菌属SGJ有潜力成为生产黑色素的新来源。
