Mittal Dinesh, Jindal Naresh, Tiwari Ashok Kumar, Khokhar Raj Singh
Animal Disease Research Center, College of Veterinary Sciences, Guru Angad Dev Veterinary and Animal Science University, Ludhiana, India.
Department of Veterinary Public Health and Epidemiology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India.
Virusdisease. 2014 Jan;25(1):114-9. doi: 10.1007/s13337-013-0183-7. Epub 2013 Nov 30.
The present study was carried out for detection and molecular characterization of fowl adenoviruses (FAdVs) associated with hydropericardium syndrome or inclusion body hepatitis in commercial broiler chickens. The FAdVs were detected in liver samples from 33 commercial broiler chicken flocks by polymerase chain reaction (PCR) using hexon gene specific primers. The restriction enzyme analysis using StyI, BsiWI, MluI, AspI, BglI and ScaI enzymes of all the 33 FAdV-positive samples revealed FAdV-4 in 10 samples, FAdV-8 in five samples, FAdV-2 and FAdV-12 in two samples each, and FAdV-5 and FAdV-6 in one sample each. Twelve samples revealed the digestion pattern for more than one serotypes with FAdV-8 and FAdV-5, FAdV-8 and FAdV-7, FAdV-8 and FAdV-6, FAdV-8 and FAdV-12 being the predominant combinations indicating mixed infection. The serotypes FAdV-2 and FAdV-5 have not been detected previously in the country. The purified PCR products of FAdVs of four samples (HR 1, HR 2, HR 3 and HR 4) were cloned and sequenced. Phylogenetic analysis revealed that FAdVs of all four samples clustered in separate groups consistent with the REA pattern. In conclusion, this study reveals the presence of FAdV-2, FAdV-4, FAdV-5, FAdV-6, FAdV-7, FAdV-8 and FAdV-12 in broiler chickens affected with hydropericardium syndrome or inclusion body hepatitis in India.
本研究旨在检测与商品肉鸡心包积水综合征或包涵体肝炎相关的禽腺病毒（FAdVs）并进行分子特征分析。使用六邻体基因特异性引物，通过聚合酶链反应（PCR）在33个商品肉鸡群的肝脏样本中检测到了FAdVs。对所有33个FAdV阳性样本使用StyI、BsiWI、MluI、AspI、BglI和ScaI酶进行限制性酶切分析，结果显示10个样本中为FAdV - 4，5个样本中为FAdV - 8，2个样本中为FAdV - 2和FAdV - 12，各有1个样本中为FAdV - 5和FAdV - 6。12个样本显示出不止一种血清型的消化模式，其中FAdV - 8与FAdV - 5、FAdV - 8与FAdV - 7、FAdV - 8与FAdV - 6、FAdV - 8与FAdV - 12为主要组合，表明存在混合感染。血清型FAdV - 2和FAdV - 5此前在该国尚未被检测到。对4个样本（HR 1、HR 2、HR 3和HR 4）的FAdVs纯化PCR产物进行了克隆和测序。系统发育分析表明，所有4个样本的FAdVs聚集在与限制性酶切分析模式一致的不同组中。总之，本研究揭示了在印度患有心包积水综合征或包涵体肝炎的肉鸡中存在FAdV - 2、FAdV - 4、FAdV - 5、FAdV - 6、FAdV - 7、FAdV - 8和FAdV - 12。
