与印度茄子花叶病相关的黄瓜花叶病毒IB亚组分离物的分子鉴定
Molecular identification of Cucumber mosaic virus isolates of subgroup IB associated with mosaic disease of eggplant in India.
作者信息
Kumar Susheel, Gautam Karmveer Kumar, Raj Shri Krishna
机构信息
Plant Molecular Virology Laboratory, CSIR-National Botanical Research Institute (NBRI), Rana Pratap Street, Lucknow, 226006 India.
出版信息
Virusdisease. 2014 Jan;25(1):129-31. doi: 10.1007/s13337-013-0174-8. Epub 2013 Nov 13.
Association of Cucumber mosaic virus (CMV) with severe mosaic disease of eggplant (Solanum melongena L.) collected from Lucknow and Kanpur, India was initially detected by host reaction and serological assay and confirmed by RT-PCR employing coat protein gene specific primers. Further, molecular identification of the virus isolates was done by cloning and sequence analysis of the complete RNA3 genome. Based on 97-99 % identities and phylogenetic relationships, the virus isolates infecting eggplant were identified as members of CMV subgroup IB.
最初通过寄主反应和血清学检测发现，从印度勒克瑙和坎普尔采集的感染严重花叶病的茄子（茄属龙葵）与黄瓜花叶病毒（CMV）有关联，并通过使用外壳蛋白基因特异性引物的RT-PCR进行了确认。此外，通过对完整RNA3基因组进行克隆和序列分析，对病毒分离株进行了分子鉴定。基于97%-99%的同一性和系统发育关系，感染茄子的病毒分离株被鉴定为CMV亚组IB的成员。
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