Chaudhary Poonam, Kaur Amritpreet, Singh Balwinder, Kumar Surender, Hallan Vipin, Nagpal Avinash Kaur
Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, India.
Post Graduate Department of Botany, Khalsa College, Amritsar, India.
Virusdisease. 2023 Mar;34(1):56-75. doi: 10.1007/s13337-022-00801-y. Epub 2022 Dec 17.
Tomato crop is known to be infected by large number of viruses across the globe causing severe losses in its yield. Accurate information on the distribution and incidence of different viruses is essential to implement virus control strategies. This study provides information on prevalence and distribution of different viruses infecting tomato crop in North-western region of India. Leaf samples of 76 symptomatic tomato and 30 symptomatic and asymptomatic plants of (weed) were collected from eight villages. DAS-ELISA and/or RT-PCR/PCR were used to detect occurrence of nineteen viruses and one viroid in tomatoes. Nine viruses viz. cucumber mosaic virus, groundnut bud necrosis virus, potato virus M, potato virus S, potato virus X, potato virus Y, tomato chlorosis virus, tomato leaf curl New Delhi virus and tomato mosaic virus were detected in 58 of 76 tomato samples. Detection of viruses was confirmed by cloning of specific amplicons followed by sequencing and submission of sequences to the GenBank database. None of the targeted pathogens were found in collected weed samples. Tomato leaf curl New Delhi virus (ToLCNDV) was the most prevalent virus (64.47%) followed by potato virus Y (PVY) (23.68%). Double, triple, quadruple and quintuple infections were also noticed. Phylogenetic analysis of nucleotide sequences was also carried out. Nine viruses infecting tomato crop from North-western region of India were detected. ToLCNDV was most prevalent with highest incidence. To the best of our knowledge, this is the first report of ToCV on tomato from India.
The online version contains supplementary material available at 10.1007/s13337-022-00801-y.
众所周知，全球范围内番茄作物会受到大量病毒的感染，导致产量严重损失。准确掌握不同病毒的分布和发病率信息对于实施病毒控制策略至关重要。本研究提供了有关印度西北地区感染番茄作物的不同病毒的流行情况和分布信息。从八个村庄采集了76株有症状的番茄叶片样本以及30株有症状和无症状的（杂草）植株样本。采用双抗夹心酶联免疫吸附测定（DAS-ELISA）和/或逆转录-聚合酶链反应（RT-PCR）/聚合酶链反应（PCR）检测番茄中19种病毒和1种类病毒的发生情况。在76份番茄样本中的58份中检测到了9种病毒，即黄瓜花叶病毒、花生芽坏死病毒、马铃薯M病毒、马铃薯S病毒、马铃薯X病毒、马铃薯Y病毒、番茄褪绿病毒、番茄曲叶新德里病毒和番茄花叶病毒。通过克隆特定扩增子，随后进行测序并将序列提交至GenBank数据库，对病毒检测结果进行了确认。在所采集的杂草样本中未发现任何目标病原体。番茄曲叶新德里病毒（ToLCNDV）是最普遍的病毒（64.47%），其次是马铃薯Y病毒（PVY）（23.68%）。还发现了双重、三重、四重和五重感染情况。对核苷酸序列也进行了系统发育分析。检测到了9种感染印度西北地区番茄作物的病毒。ToLCNDV最为普遍，发病率最高。据我们所知，这是印度首次关于番茄褪绿病毒（ToCV）的报道。
在线版本包含可在10.1上获取的补充材料。1007/s13之337 - 022 - 00801 - y。
