Paul R, Herbert J M, Maffrand J P, Lansen J, Modat G, Pereillo J M, Gordon J L
Thromb Res. 1987 Jun 15;46(6):793-801. doi: 10.1016/0049-3848(87)90071-5.
Pentosan polysulphate (PPS; SP 54; Hemoclar), a highly sulphated semi-synthetic polysaccharide of MW 4.7 kD, was tested in vitro as an inhibitor of rabbit aortic smooth muscle cell (SMC) proliferation and its effects were compared with those of dextran sulphate, laminarin sulphate and heparin fractions. When added to the cell cultures simultaneously with foetal calf serum, all sulphated polysaccharides inhibited cell growth. PPS was five fold more active than heparin and its low molecular weight fractions. A high molecular weight fraction of PPS was the most active inhibitor (IC50: 2.7 micrograms/ml vs 45 micrograms/ml for PPS, and 241 micrograms/ml for heparin). When PPS and heparin were pre-incubated with the cells for three days, then removed before foetal calf serum was added to stimulate growth, their inhibitory effects were comparable with those seen when the compounds were present throughout the growth phase, suggesting that they exert their effect by binding to the cell surface, and not by interacting with the growth factors in serum.
戊聚糖多硫酸盐（PPS；SP 54；Hemoclar）是一种分子量为4.7 kD的高度硫酸化半合成多糖，在体外作为兔主动脉平滑肌细胞（SMC）增殖抑制剂进行了测试，并将其效果与硫酸葡聚糖、硫酸海带多糖和肝素组分进行了比较。当与胎牛血清同时添加到细胞培养物中时，所有硫酸化多糖均抑制细胞生长。PPS的活性比肝素及其低分子量组分高五倍。PPS的高分子量组分是最有效的抑制剂（IC50：PPS为2.7微克/毫升，肝素为45微克/毫升，肝素为241微克/毫升）。当PPS和肝素与细胞预孵育三天，然后在添加胎牛血清以刺激生长之前去除时，它们的抑制作用与在整个生长阶段都存在这些化合物时观察到的抑制作用相当，这表明它们通过与细胞表面结合发挥作用，而不是通过与血清中的生长因子相互作用发挥作用。
