Boon M E, Kok L P, Moorlag H E, Gerrits P O, Suurmeijer A J
Stain Technol. 1987 Jul;62(4):257-66. doi: 10.3109/10520298709108004.
Staining plastic sections with the Romanowsky-Giemsa method is both time-consuming and difficult. This paper reports how the staining time can be reduced to 25 min using microwave irradiation of the staining solution. It is shown that staining results depend on the fixative used, staining temperature, dye concentration and pH of the staining solution as well as on several parameters of the microwave irradiation technique. The staining patterns are improved when compared with those obtained by conventional staining of plastic sections. The colors are more brilliant and greater contrasts are observed. Basophilia, polychromasia, and orthochromasia accompanying red cell maturation are more pronounced. For white cell maturation the initial appearance of specific granules (neutrophil, basophil, and eosinophil) is more evident. Thus, cell classification is easily accomplished using the described technique. It is suggested that microwave-stimulated staining be considered for routine use.
用罗曼诺夫斯基-吉姆萨法对塑料切片进行染色既耗时又困难。本文报道了如何通过对染色液进行微波辐照将染色时间缩短至25分钟。结果表明，染色结果取决于所用固定剂、染色温度、染料浓度、染色液的pH值以及微波辐照技术的几个参数。与传统的塑料切片染色相比，染色模式有所改善。颜色更鲜艳，对比度更高。红细胞成熟过程中的嗜碱性、多色性和正染性更为明显。对于白细胞成熟，特定颗粒（中性粒细胞、嗜碱性粒细胞和嗜酸性粒细胞）的初始出现更为明显。因此，使用所述技术很容易完成细胞分类。建议考虑将微波刺激染色用于常规用途。
