Kayser K, Bubenzer J
Institute of Pathology, Lutherian Hospital, Oberhausen, FRG.
Histochem J. 1990 Jun-Jul;22(6-7):365-70. doi: 10.1007/BF01003171.
The use and practicability of microwave-assisted staining procedures in routine histopathology over more than three years has been evaluated. A domestic microwave oven was used to speed up the following staining procedures: Haematoxylin-Eosin (for frozen sections), Romanowsky-Giemsa, Periodic acid-Schiff (PAS), Ziehl-Neelson, Papanicolaou, Feulgen and Grocott--stain on buffered formalin fixed sections or cytologic smears. These staining procedures can be made highly reproducible providing; (1) Staining vessels are placed in the same position inside the oven; (2) Accurate timing in seconds is observed. Microwave-assisted staining procedures are equal to or even superior to those of the standard methods. Staining times can be reduced to 2%-10% of the conventional staining procedures. The basic staining protocols are presented.
对微波辅助染色程序在常规组织病理学中三年多的使用情况及实用性进行了评估。使用一台家用微波炉来加速以下染色程序：苏木精-伊红染色（用于冰冻切片）、罗曼诺夫斯基-吉姆萨染色、过碘酸-希夫染色（PAS）、齐尔-尼尔森染色、巴氏染色、福尔根染色和格罗科特染色——对用缓冲福尔马林固定的切片或细胞学涂片进行染色。只要满足以下条件，这些染色程序就能具有高度可重复性：（1）染色容器放置在烤箱内的同一位置；（2）精确到秒计时。微波辅助染色程序等同于甚至优于标准方法。染色时间可缩短至传统染色程序的2%至10%。文中给出了基本的染色方案。
