萘啶酸对大肠杆菌K-12细胞的穿透作用。
Penetration of nalidixic acid into Escherichia coli K-12 cells.
作者信息
Hrebenda J, Brzostek K, Heleszko H
出版信息
Acta Microbiol Pol. 1987;36(1-2):67-72.
Transport of nalidixic acid (NAL) into Escherichia coli cells subjected to osmotic shock, permeabilised with toluene or treated with DNP, CCCP or EDTA, was studied. It was found that osmotic shock and protonophores do not inhibit the transport of [3H]NAL, however, the transport of [3H]DAP and [3H]glucose is reduced. EDTA and toluene enhance penetration of [3H]NAL. This effect is, however, abolished in the presence of Mg++ ions. It is suggested that NAL penetrates into the cell by simple or facilitated diffusion and that the outer membrane of E. coli is the penetration barrier for the drug.
研究了萘啶酸（NAL）在经渗透压休克、用甲苯通透处理或用二硝基苯酚（DNP）、羰基氰氯苯腙（CCCP）或乙二胺四乙酸（EDTA）处理的大肠杆菌细胞中的转运情况。发现渗透压休克和质子载体并不抑制[³H]NAL的转运，然而，[³H]二氨基庚二酸（DAP）和[³H]葡萄糖的转运却减少了。EDTA和甲苯可增强[³H]NAL的渗透。然而，在Mg²⁺离子存在时这种效应会消失。提示NAL通过简单扩散或易化扩散进入细胞，且大肠杆菌的外膜是该药物的渗透屏障。
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