Nuclear Institute for Agriculture & Biology, Jhang Road, PO Box 128, Faisalabad, Pakistan.
World J Microbiol Biotechnol. 1990 Mar;6(1):46-52. doi: 10.1007/BF01225354.
Diazotrophs, especially of genusAzospirillum were readily isolated from roots of many plants using semi-solid nitrogen free malate medium (NFM). These isolates formed fine, white sub-surface pellicle in nitrogen-free malate medium within 24h, which gradually moved to the surface, and exhibited high acetylene reduction rates. Using selected cultural and biochemical tests, most of the isolates were identified asAzospirillum brasilense. Four isolates from Kallar grass root surface and one isolate fromAtriplex root interior formed phenotypically a homogenous group. It shared many characteristics with the species of genusAzospirillum except shape. All the biochemical tests performed, categorized them withA. brasilense. However, the shape and the protein profile on SDS polyacrylamide gel electrophoretograms suggested that the group of these five isolates is clearly distinct and differs widely from all the type strains, belonging to various genera.
固氮菌，特别是节杆菌属，很容易从许多植物的根部分离出来，使用半固态无氮苹果酸盐培养基（NFM）。这些分离物在无氮苹果酸盐培养基中 24 小时内形成细小的白色亚表面菌膜，逐渐移动到表面，并表现出高的乙炔还原率。使用选定的文化和生化试验，大多数分离物被鉴定为巴西固氮螺菌。从 Kallar 草根表面分离的四个分离物和从滨藜根内部分离的一个分离物在表型上形成了同质群体。它与节杆菌属的物种除了形状之外，有许多共同的特征。所有进行的生化试验将它们归类为巴西固氮螺菌。然而，形态和 SDS 聚丙烯酰胺凝胶电泳图谱上的蛋白质图谱表明，这五个分离物的群体明显不同，与属于不同属的各种类型菌株有很大的不同。
