Division of Biochemistry, Instituto de Investigaciones Biologicas Clemente Estable (IIBCE), Avda. Italia 3318, Montevideo, Uruguay.
World J Microbiol Biotechnol. 1990 Jun;6(2):121-6. doi: 10.1007/BF01200931.
Introduced clovers (Trifolium repens andTrifolium subterraneum) fall to persist more than two years in Uruguayan improved pastures. Three strains of naturalizedRhizobium leguminosarum bvtrifolii, isolated from persistent clover pastures inoculated several years ago, and two commercial inoculant strains have been identified. Serological techniques (enzyme-linked immunosorbent assay-ELISA) and total cell protein pattern have been selected as sensitive and reliable methods for strain identification. Indirect ELISA was suitable for detecting rhizobia in cultures or in nodules. Two of the naturalized strains had distinct protein profiles but they could not be differentiated by the immunological method with a polyclonal antibody.
引入的三叶草（白车轴草和紫花苜蓿）在乌拉圭改良的牧场上能够持续存在超过两年。从几年前接种持久性三叶草牧场中分离出的三种天然根瘤菌 Rhizobium leguminosarum bvtrifolii 菌株和两种商业接种剂菌株已被鉴定出来。血清学技术（酶联免疫吸附试验-ELISA）和总细胞蛋白图谱已被选为菌株鉴定的敏感可靠方法。间接 ELISA 适用于检测培养物或根瘤中的根瘤菌。两种天然化的菌株具有明显不同的蛋白质图谱，但它们不能通过用多克隆抗体的免疫学方法来区分。
