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简化酶联免疫吸附试验用于常规鉴定根瘤菌抗原。

Simplified Enzyme-Linked Immunosorbent Assay for Routine Identification of Rhizobium japonicum Antigens.

机构信息

Soil Science Department, North Carolina State University, Raleigh, North Carolina 27695-7619.

出版信息

Appl Environ Microbiol. 1985 Apr;49(4):1010-3. doi: 10.1128/aem.49.4.1010-1013.1985.

DOI:10.1128/aem.49.4.1010-1013.1985
PMID:16346765
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC238490/
Abstract

A simple, reliable, and flexible modification of the indirect enzyme-linked immunosorbent assay was developed for the identification of Rhizobium japonicum antigens from cultures and nodules. The procedure emphasizes efficient use of time and reagents, adaptability to variously equipped laboratories, and maintenance of sensitivity levels that are adequate for ecological studies.

摘要

本研究开发了一种简单、可靠且灵活的间接酶联免疫吸附测定法（ELISA）改良方法，用于鉴定来自培养物和根瘤的根瘤菌抗原。该方法强调了有效利用时间和试剂、适应各种不同设备实验室的能力，以及保持足够用于生态研究的灵敏度水平。

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2
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Appl Environ Microbiol. 1979 Mar;37(3):642-6. doi: 10.1128/aem.37.3.642-646.1979.
3
IMMUNE DIFFUSION ANALYSIS OF THE EXTRACELLULAR SOLUBLE ANTIGENS OF TWO STRAINS OF RHIZOBIUM MELILOTI.两株苜蓿根瘤菌胞外可溶性抗原的免疫扩散分析
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4
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5
Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G.酶联免疫吸附测定（ELISA）。免疫球蛋白G的定量测定。
Immunochemistry. 1971 Sep;8(9):871-4. doi: 10.1016/0019-2791(71)90454-x.
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J Immunol. 1972 Jul;109(1):129-35.
7
Rhizobium strain identification in Arachis hypogaea nodules by enzyme-linked immunosorbent assay (ELISA).通过酶联免疫吸附测定（ELISA）对花生根瘤中的根瘤菌菌株进行鉴定。
Can J Microbiol. 1978 Dec;24(12):1537-43. doi: 10.1139/m78-245.