1] Centre of Genomics and Bioinformatics, Academy of Sciences of Uzbekistan, Ministry of Agriculture & Water Resources of Uzbekistan, and 'Uzpakhtasanoat' Association, University street-2, Kibray region, Tashkent 111215, Uzbekistan [2].
USDA-ARS, Crop Science Research Laboratory, Genetics and Precision Agriculture, P. O. Box 5367, 812 Highway 12E, Mississippi State, Mississippi 39762, USA.
Nat Commun. 2014;5:3062. doi: 10.1038/ncomms4062.
Simultaneous improvement of fibre quality, early-flowering, early-maturity and productivity in Upland cotton (G. hirsutum) is a challenging task for conventional breeding. The influence of red/far-red light ratio on the fibre length prompted us to examine the phenotypic effects of RNA interference (RNAi) of the cotton PHYA1 gene. Here we show a suppression of up to ~70% for the PHYA1 transcript, and compensatory overexpression of up to ~20-fold in the remaining phytochromes in somatically regenerated PHYA1 RNAi cotton plants. Two independent transformants of three generations exhibited vigorous root and vegetative growth, early-flowering, significantly improved upper half mean fibre length and an improvement in other major fibre characteristics. Small decreases in lint traits were observed but seed cotton yield was increased an average 10-17% compared with controls. RNAi-associated phenotypes were heritable and transferable via sexual hybridization. These results should aid in the development of early-maturing and productive Upland cultivars with superior fibre quality.
在常规育种中，同时提高陆地棉（G. hirsutum）纤维品质、早花、早熟和生产力是一项具有挑战性的任务。红光/远红光比值对纤维长度的影响促使我们研究棉花 PHYA1 基因的 RNA 干扰（RNAi）的表型效应。在这里，我们展示了 PHYA1 转录本的抑制高达约 70%，而在体细胞再生的 PHYA1 RNAi 棉花植物中，剩余的光敏色素的补偿性过表达高达约 20 倍。经过三代的两个独立转化体表现出旺盛的根和营养生长、早花、上半均值纤维长度显著提高以及其他主要纤维特性得到改善。观察到 lint 性状略有下降，但与对照相比，皮棉产量平均增加了 10-17%。与对照相比，RNAi 相关表型是可遗传的，并可通过有性杂交转移。这些结果应该有助于开发具有优良纤维品质的早熟和高产陆地棉品种。
