Saleem Muhammad Sulyman, Khan Sultan Habibullah, Ahmad Aftab, Rana Iqrar Ahmad, Naveed Zunaira Afzal, Khan Azeem Iqbal
Centre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture Faisalabad, Faisalabad, Pakistan.
Center for Advanced Studies in Agriculture and Food Security (CAS-AFS), University of Agriculture Faisalabad, Faisalabad, Pakistan.
Front Genome Ed. 2024 Sep 12;6:1401088. doi: 10.3389/fgeed.2024.1401088. eCollection 2024.
Cotton is globally known for its high-priority cellulose-rich natural fiber. In addition to providing fiber for the textile industry, it is an important source material for edible oil, livestock feed, and fuel products. Global warming and the growing population are the major challenges to the world's agriculture and the potential risks to food security. In this context, improving output traits in cotton is necessary to achieve sustainable cotton production. During the last few years, high throughput omics techniques have aided in identifying crucial genes associated with traits of cotton fiber, seed, and plant architecture which could be targeted with more precision and efficiency through the CIRPSR/Cas-mediated genome editing technique. The various CRISPR/Cas systems such as CRISPR/Cas9, CRISPR/nCas9, and CRISPR/Cas12a have been employed to edit cotton genes associated with a wide range of traits including fiber length, flowering, leaf colour, rooting, seed oil, plant architecture, gossypol content, somatic embryogenesis, and biotic and abiotic stresses tolerance, highlighting its effectiveness in editing the cotton genome. Thus, CRISPR/Cas-mediated genome editing has emerged as a technique of choice to tailor crop phenotypes for better yield potential and environmental resilience. The review covers a comprehensive analysis of cotton phenotypic traits and their improvement with the help of the latest genome editing tools to improve fiber, food, feed, and fuel-associated genes of cotton to ensure food security.
棉花以其富含纤维素的高优先级天然纤维而闻名于世。除了为纺织工业提供纤维外，它还是食用油、牲畜饲料和燃料产品的重要原料。全球变暖和人口增长是世界农业面临的主要挑战，也是粮食安全的潜在风险。在这种背景下，提高棉花的产量性状对于实现棉花可持续生产至关重要。在过去几年中，高通量组学技术有助于识别与棉花纤维、种子和植株结构性状相关的关键基因，通过CIRPSR/Cas介导的基因组编辑技术可以更精确、高效地对这些基因进行靶向操作。各种CRISPR/Cas系统，如CRISPR/Cas9、CRISPR/nCas9和CRISPR/Cas12a，已被用于编辑与多种性状相关的棉花基因，包括纤维长度、开花、叶片颜色、生根、种子油、植株结构、棉酚含量、体细胞胚胎发生以及对生物和非生物胁迫的耐受性，凸显了其在编辑棉花基因组方面的有效性。因此，CRISPR/Cas介导的基因组编辑已成为一种首选技术，用于塑造作物表型，以获得更好的产量潜力和环境适应能力。这篇综述全面分析了棉花的表型性状，以及借助最新的基因组编辑工具对其进行改良，以改善棉花中与纤维、食品、饲料和燃料相关的基因，确保粮食安全。
