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(3)H 标记多聚尿嘧啶核苷酸与菜豆 DNA 的杂交

The hybridisation of [(3)H]poly(uridylic) acid to DNA from Phaseolus aureus Roxb.

机构信息

Department of Physiology and Environmental Studies, University of Nottingham, School of Agriculture, Sutton Bonington, LE12 5RD, Loughborough, U.K..

出版信息

Planta. 1975 Jan;127(1):87-91. doi: 10.1007/BF00388866.

Abstract

[5-(3)H]poly(uridylic) acid hybridises to nuclear DNA, suggesting the DNA contains poly (deoxyadenylic) acid sequences. These regions do not appear to be clustered in the DNA. Although the amount of DNA involved is small it is sufficient for thousands of sequences. Thermal dissociation of the hybrids shows that a relatively small number of hydrogen bonds is involved in each and it is concluded that these sequences are relatively short. In view of the fact that much longer poly(adenylic) acid sequences are found in plant RNA it is concluded that these are added after transcription.

摘要

[5-(3)H]聚(尿嘧啶核苷酸)与核 DNA 杂交,表明 DNA 中含有聚(脱氧腺苷酸)序列。这些区域似乎没有在 DNA 中聚集。尽管涉及的 DNA 量很小,但足以容纳数千个序列。杂交体的热解离表明每个序列涉及的氢键数量相对较少,因此可以得出结论,这些序列相对较短。鉴于在植物 RNA 中发现了更长的聚(腺苷酸)序列,因此可以得出结论,这些序列是在转录后添加的。

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