用放射性多聚尿嘧啶核苷 hybridization 测量植物多聚腺苷酸。
The measurement of plant polyadenylic acid by hybridisation with radioactive polyuridylic acid.
机构信息
Department of Physiology and Environmental Studies, University of Nottingham School of Agriculture, Sutton Bonington, LE12 5RD, Loughborough, UK.
出版信息
Planta. 1976 Jan;131(1):75-9. doi: 10.1007/BF00387348.
Saturation hybridisation of polyadenylic acid with [(3)H]polyuridylic acid is described. Under conditions of [(3)H]poly(U) excess, poly(A) is detected in the RNA of a number of higher plants. The ribonuclease resistant hybrids melt sharply when subjected to thermal denaturation. Plant RNA which contains poly(A) sequences detected by [(3)H]poly(U) hybridisation is polydisperse in molecular weight. Data presented shows that the amount of poly(A) in plant RNA is variable. This technique is useful for the qualitative and quantitative detection of poly(A) sequences in higher plant RNA.
本文描述了聚腺苷酸与 [(3)H] 聚尿嘧啶核苷酸的饱和杂交。在 [(3)H] 聚(U) 过量的条件下，许多高等植物的 RNA 中都检测到了聚(A)。当进行热变性时，核糖核酸酶抗性杂交体急剧熔化。通过 [(3)H] 聚(U) 杂交检测到含有聚(A)序列的植物 RNA 在分子量上是多分散的。所提供的数据表明，植物 RNA 中的聚(A)含量是可变的。该技术可用于定性和定量检测高等植物 RNA 中的聚(A)序列。
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